This phospho-Oct4 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS236 of human OCT4.
Titration of the phospho-Oct4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Dot blot: 1:500
限制
仅限研究用
状态
Liquid
缓冲液
In 1X PBS pH 7.4 with 0.09 % sodium azide
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Aliquot the phospho-Oct4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
抗原
OCT4 (POU5F1)
(POU Class 5 Homeobox 1 (POU5F1))
别名
Oct4
背景
OCT4 encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6,22)(p21,q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.