PGK1 抗体 (AA 117-145)
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北京 101111
Quick Overview for PGK1 抗体 (AA 117-145) (ABIN3032224)
抗原
See all PGK1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 117-145
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交叉反应 (详细)
- Expected species reactivity: Primate, Rat
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纯化方法
- Purified
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免疫原
- A portion of amino acids 117-145 from the human protein was used as the immunogen for this PGK1 antibody.
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亚型
- Ig Fraction
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应用备注
- Titration of the PGK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:100,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- In 1X PBS, pH 7.4, with 0.09 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Aliquot the PGK1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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- PGK1 (Phosphoglycerate Kinase 1 (PGK1))
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别名
- PGK1
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背景
- Also known as ATP:3-phosphoglycerate 1-phosphotransferase (EC 2.7.2.3), this major enzyme in glycolysis catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate, generating one molecule of ATP. New blood vessel formation or angiogenesis is critical for tumor expansion and metastasis. Lay et al. (2000) showed that the plasmin reductase isolated from conditioned medium of fibrosarcoma cells is the glycolytic enzyme phosphoglycerate kinase. They concluded that phosphoglycerate kinase not only functions in glycolysis but is secreted by tumor cells and participates in the angiogenic process as a disulfide reductase.
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UniProt
- P00558
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途径
- Cellular Glucan Metabolic Process
抗原
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