Nanog 抗体 (pSer71)
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北京 101111
Quick Overview for Nanog 抗体 (pSer71) (ABIN3031940)
抗原
See all Nanog (NANOG) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer71
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交叉反应 (详细)
- Expected species reactivity: Primate
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纯化方法
- Antigen affinity purified
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免疫原
- This p-NANOG antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pS71 of human Nanog.
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亚型
- Ig Fraction
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应用备注
- Titration of the p-NANOG antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Immunofluorescence: 1:10-1:50,Dot blot: 1:500
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- In 1X PBS, pH 7.4, with 0.09 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Aliquot the p-NANOG antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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- Nanog (NANOG) (Nanog Homeobox (NANOG))
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别名
- NANOG
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背景
- NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cels proliferation and self-renewal. It imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. This protein blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. NANOG acts as a transcriptional activator or repressor. It binds optimally to the DNA consensus sequence 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, this protein promotes cells to enter into S phase and proliferation.
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UniProt
- Q9H9S0
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途径
- Stem Cell Maintenance
抗原
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