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LIN28B 抗体 (AA 1-30)

The 兔 多克隆 anti-LIN28B antibody is suitable to detect LIN28B in samples from 人. It has been validated for WB, ELISA, FACS, IHC 和 IF.
产品编号 ABIN3031628
发货至: 中国
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Room 801-803
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Quick Overview for LIN28B 抗体 (AA 1-30) (ABIN3031628)

抗原

See all LIN28B 抗体
LIN28B (Lin-28 Homolog B (LIN28B))

适用

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宿主

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克隆类型

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多克隆

标记

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This LIN28B antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)
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    AA 1-30

    纯化方法

    Purified

    免疫原

    A portion of amino acids 1-30 from the human protein was used as the immunogen for this LIN28B antibody.

    亚型

    Ig Fraction
  • 应用备注

    Titration of the LIN28B antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Immunofluorescence: 1:100,Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    In 1X PBS, pH 7.4, with 0.09 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Aliquot the LIN28B antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
  • 抗原

    LIN28B (Lin-28 Homolog B (LIN28B))

    别名

    LIN28B

    背景

    Lin-28 homolog B (LIN28B) is overexpressed in hepatocellular carcinoma. The heterochronic gene lin-28 is a key regulator of developmental timing in the nematode Caenorhabditis elegans. Similar with lin-28 proteins, LIN28B conserves a cold shock domain and a pair of CCHC zinc finger domains. Phylogenetic analysis suggests that they might arise as a result of duplication from an ancestral gene. Overexpression of LIN28B was noted in most HCC cell lines and clinical samples. A short LIN28B isoform was also identified in non-tumor liver tissue and fetal liver. Although predominantly localized in the cytoplasm, LIN28B protein shows cell cycle-dependent nuclear translocation in Huh7 cells. Induced expression of exogenous LIN28B in a tet-off cell line promoted cancer cell proliferation.

    UniProt

    Q6ZN17
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