ARF1 抗体 (AA 80-106)
Quick Overview for ARF1 抗体 (AA 80-106) (ABIN3029877)
抗原
See all ARF1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 80-106
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交叉反应 (详细)
- Expected species reactivity: Mouse, Rat, Bovine, Primate, Xenopus
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纯化方法
- Antigen affinity purified
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免疫原
- A portion of amino acids 80-106 from the human protein was used as the immunogen for this ARF1 antibody.
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亚型
- Ig Fraction
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应用备注
- Titration of the ARF1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- In 1X PBS, pH 7.4, with 0.09 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Aliquot the ARF1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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- ARF1 (ADP-Ribosylation Factor 1 (ARF1))
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别名
- ARF1
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背景
- ARF1 is a GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity, the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasicity of excitatory synapses and spine shrinkage during long-term depression (LTD). [UniProt]
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UniProt
- P84077
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途径
- Transition Metal Ion Homeostasis, Inositol Metabolic Process
抗原
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