Titration of the anti-HA antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000, Immunoprecipitation: published application
限制
仅限研究用
状态
Liquid
缓冲液
In 1X PBS pH 7.4 with 0.09 % sodium azide
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Aliquot the anti-HA antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Epitope tags consisting of short sequences recognized by well-characterizated monoclonal antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYA) and Myc Tag (AEEQKLISEEDLLRKRREQLKHKLE), recognized by monoclonal antibody clones 12CA5 and 9E10, respectively, are illustrative examples. NSJ's anti-HA monoclonal antibody (Clone 12CA5) provides a simple solution to detect the expression of an HA-tagged protein in cells.