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Recombinant Phosphotyrosine 抗体

The 兔 单克隆 anti-Phosphotyrosine antibody (Clone RM111) (ABIN4953419) specifically detects Phosphotyrosine in WB, IP, IHC, ICC 和 ChIP.
产品编号 ABIN4953419
发货至: 中国
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中国
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Recombinant Phosphotyrosine 抗体 (ABIN4953419)

抗原

Phosphotyrosine

抗体类型

Recombinant Antibody

宿主

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克隆类型

  • 132
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单克隆

标记

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This Phosphotyrosine antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP)

克隆位点

RM111
  • 原理

    Recombinant Phosphotyrosine Antibody

    产品特性

    This recombinant Phosphotyrosine antibody reacts to tyrosine-phosphorylated proteins. No cross reactivity with nonphosphorylated tyrosine, phosphoserine, and phosphorthreonine seen.

    纯化方法

    Protein A purified from animal origin-free supernatant

    免疫原

    Phosphotyrosine-BSA conjugate was used as the immunogen for this recombinant Phosphotyrosine antibody.

    亚型

    IgG
  • 应用备注

    The stated application concentrations are suggested starting points. Titration of the recombinant Phosphotyrosine antibody may be required due to differences in protocols and secondary/substrate sensitivity.

    1. A pH 6 Citrate buffer or pH 9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    1 mg/mL in PBS with 50 % glycerol, 1 % BSA and 0.09 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Store the recombinant Phosphotyrosine antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
  • 抗原

    Phosphotyrosine

    物质类

    Amino Acid

    背景

    This recombinant Phosphotyrosine antibody reacts to tyrosine-phosphorylated proteins. No cross reactivity with nonphosphorylated tyrosine, phosphoserine, and phosphorthreonine seen.
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