Recombinant 5-Hydroxymethylcytosine 抗体
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- 抗原 See all 5-Hydroxymethylcytosine (5-hmC) products
- 5-Hydroxymethylcytosine (5-hmC)
- 抗体类型
- Recombinant Antibody
- 适用
- 请咨询
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宿主
- 兔
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克隆类型
- 单克隆
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标记
- This 5-Hydroxymethylcytosine antibody is un-conjugated
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应用范围
- ELISA, Dot Blot (DB), Hydroxymethylated DNA immunoprecipitation (hmeDIP), Immunocytochemistry (ICC), Immunohistochemistry (IHC)
- 产品特性
- This recombinant 5hmC antibody reacts to 5-hydroxymethylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and methylcytosine in DNA.
- 纯化方法
- Protein A purified from animal origin-free supernatant
- 免疫原
- BSA-conjugated 5-hydroxymethylcytosine was used as the immunogen for this recombinant 5hmC antibody.
- 克隆位点
- RM236
- 亚型
- IgG
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anti-5-Hydroxymethylcytosine (5-hmC) antibody
5-hmC DB, hmeDIP, ICC, IF, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-5-Hydroxymethylcytosine (5-hmC) antibody5-hmC 适用: 人, 小鼠 DB, ICC, MeDIP, IF, IHC 宿主: 小鼠 Monoclonal 59-1 unconjugated
anti-5-Hydroxymethylcytosine (5-hmC) antibody5-hmC DB, hmeDIP, ICC, IF 宿主: 小鼠 Monoclonal 59-1 unconjugated
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- 应用备注
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The stated application concentrations are suggested starting points. Titration of the recombinant 5hmC antibody may be required due to differences in protocols and secondary/substrate sensitivity.
1. A pH 6 Citrate buffer or pH 9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.\. Dot Blot: 0.2-1 μg/mL,ELISA: 0.1-1 μg/mL,Immunohistochemistry: 0.1-1 μg/mL (1),Immunocytochemistry: 0.5-2 μg/mL,hMeDIP: 0.2 μg/mL- 2 μg/mL
- 限制
- 仅限研究用
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- 缓冲液
- 1 mg/mL in PBS with 50 % glycerol, 1 % BSA and 0.09 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store the recombinant 5hmC antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol).
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- 抗原
- 5-Hydroxymethylcytosine (5-hmC)
- 别名
- 5-Hydroxymethylcytosine (5hmC) (5-hmC 产品)
- 物质类
- Chemical
- 背景
- This recombinant 5hmC antibody reacts to 5-hydroxymethylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and methylcytosine in DNA.
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