5-Hydroxymethylcytosine 抗体
Our Local Distributor
北京 101111
Quick Overview for 5-Hydroxymethylcytosine 抗体 (ABIN7540500)
抗原
适用
宿主
克隆类型
标记
应用范围
-
-
Supplier Product No.
- 200-301-v06
-
Supplier
- Rockland
-
原理
- 5-hmc Antibody
-
交叉反应 （详细）
- Cross-reactivity with 5hmC from other sources has not been determined.
-
纯化方法
- Anti-5hmC Antibody was purified by Protein A chromatography.
-
过滤
- Sterile filtered
-
免疫原
- Anti-5hmC Antibody was produced in mice by repeated immunization with 5-hydroxymethlcytosine.
-
亚型
- IgG1 kappa
-
-
anti-5-Hydroxymethylcytosine (5-hmC) antibody
5-hmC DB, ICC, hmeDIP, IF, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-5-Hydroxymethylcytosine (5-hmC) antibody5-hmC 适用: 人, 小鼠 DB, MeDIP, ICC, IF, IHC 宿主: 小鼠 Monoclonal 59-1 unconjugated
anti-5-Hydroxymethylcytosine (5-hmC) antibody5-hmC 适用: 人 ELISA, DB, ICC, hmeDIP, IHC 宿主: 兔 Monoclonal RM236 unconjugated Recombinant Antibody
anti-5-Hydroxymethylcytosine (5-hmC) antibody5-hmC DB, ICC, hmeDIP, IF 宿主: 小鼠 Monoclonal 59-1 unconjugated
-
-
应用备注
-
ELISA_Dilution: 1:500
IF_Microscopy_Dilution: User Optimized
Other: hMeDIP: 2.5 μg per IP. Dot Blot: 2 μg/mL
-
说明
-
Suggested Applications: hMeDIP: 2.5μg per IP. Dot Blot: 2 μg/ml
Anti-5hmC Antibody is tested for Dot Blotting, ELISA, and Hydroxymethylated DNA Immunoprecipitation Sequencing. Specific conditions for reactivity should be optimized by the end user. -
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
缓冲液
-
Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.05 % (w/v) Sodium Azide -
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
-
有效期
- 12 months
-
-
- 5-Hydroxymethylcytosine (5-hmC)
-
别名
- 5-hydroxymethylcytosine
-
物质类
- Chemical
-
背景
- 5-hydroxymethylcytosine,5-hydroxymethylcytosine (5-hmC) has been recently discovered in mammalian DNA. This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. So far, the 5-hmC bases have been identified in Purkinje neurons, in granule cells and embryonic stem cells where they are present at high levels (up to 0,6 % of total nucleotides in Purkinje cells). Preliminary results indicate that 5-hmC may have important roles distinct from 5-mC. Although its precise role has still to be shown, early evidence suggests a few putative mechanisms that could have big implications in epigenetics: 5-hydroxymethylcytosine may well represent a new pathway to demethylate DNA involving a repair mechanism converting 5-hmC to cytosine and, as such open up entirely new perspectives in epigenetic studies. Due to the structural similarity between 5-mC and 5-hmC, these bases are experimentally almost indistinguishable. Recent articles demonstrated that the most common approaches (e.g. enzymatic approaches, bisulfite sequencing) do not account for 5-hmC. The development of the affinity-based technologies appears to be the most powerful way to differentially and specifically enrich 5-mC and 5-hmC sequences. Anti-5hmC Antibody is ideal for research in Epigenetics and Gene Expression.
抗原
-
