Recombinant 5-Hydroxymethylcytosine 抗体
Quick Overview for Recombinant 5-Hydroxymethylcytosine 抗体 (ABIN4953286)
抗原
抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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产品特性
- This recombinant 5hmC antibody reacts to 5-hydroxymethylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and methylcytosine in DNA.
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纯化方法
- Protein A purified from animal origin-free supernatant
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免疫原
- BSA-conjugated 5-hydroxymethylcytosine was used as the immunogen for this recombinant 5hmC antibody.
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亚型
- IgG
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anti-5-Hydroxymethylcytosine (5-hmC) antibody
5-hmC DB, hmeDIP, ICC, IF, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-5-Hydroxymethylcytosine (5-hmC) antibody5-hmC 适用: 人, 小鼠 DB, ICC, MeDIP, IF, IHC 宿主: 小鼠 Monoclonal 59-1 unconjugated
anti-5-Hydroxymethylcytosine (5-hmC) antibody5-hmC DB, hmeDIP, ICC, IF 宿主: 小鼠 Monoclonal 59-1 unconjugated
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应用备注
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The stated application concentrations are suggested starting points. Titration of the recombinant 5hmC antibody may be required due to differences in protocols and secondary/substrate sensitivity.
1. A pH 6 Citrate buffer or pH 9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.\. Dot Blot: 0.2-1 μg/mL,ELISA: 0.1-1 μg/mL,Immunohistochemistry: 0.1-1 μg/mL (1),Immunocytochemistry: 0.5-2 μg/mL,hMeDIP: 0.2 μg/mL- 2 μg/mL
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限制
- 仅限研究用
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缓冲液
- 1 mg/mL in PBS with 50 % glycerol, 1 % BSA and 0.09 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store the recombinant 5hmC antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol).
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- 5-Hydroxymethylcytosine (5-hmC)
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别名
- 5-Hydroxymethylcytosine (5hmC)
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物质类
- Chemical
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背景
- This recombinant 5hmC antibody reacts to 5-hydroxymethylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and methylcytosine in DNA.
抗原
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