5-Hydroxymethylcytosine 抗体
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北京 101111
Quick Overview for 5-Hydroxymethylcytosine 抗体 (ABIN2668979)
抗原
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- 5-Hydroxymethylcytosine (5-hmC) antibody (mAb) (Clone 59.1)
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纯化方法
- Protein G Chromatography
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免疫原
- This 5-Hydroxymethylcytosine antibody was raised against 5-hydroxymethylcytidine conjugated to KLH and recognizes 5-hydroxymethylcytosine.
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亚型
- IgG2a
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anti-5-Hydroxymethylcytosine (5-hmC) antibody
5-hmC DB, MeDIP, IHC, IF, ICC, FACS 宿主: 兔 Polyclonal unconjugated
anti-5-Hydroxymethylcytosine (5-hmC) antibody5-hmC 适用: 人, 小鼠 DB, MeDIP, IHC, IF, ICC 宿主: 小鼠 Monoclonal 59-1 unconjugated
anti-5-Hydroxymethylcytosine (5-hmC) antibody5-hmC 适用: 人 DB, ELISA, IHC, ICC, hmeDIP 宿主: 兔 Monoclonal RM236 unconjugated Recombinant Antibody
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应用备注
- MeDIP: 1 - 2 μg per IP MeDIP-chip: 10 μg per IP DB: 0.2 μg/mL dilution
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限制
- 仅限研究用
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浓度
- 1 μg/μL
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缓冲液
- Purified IgG in PBS, 30 % glycerol and 0.035 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage
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储存条件
- -20 °C
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储存方法
- Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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有效期
- 24 months
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: "Maternal low-protein diet affects epigenetic regulation of hepatic mitochondrial DNA transcription in a sex-specific manner in newborn piglets associated with GR binding to its promoter." in: PLoS ONE, Vol. 8, Issue 5, pp. e63855, (2013) (PubMed).
: "5'-Methylcytosine and 5'-hydroxymethylcytosine each provide epigenetic information to the mouse zygote." in: PLoS ONE, Vol. 8, Issue 5, pp. e63689, (2013) (PubMed).
: "Glucocorticoid receptor is involved in the breed-dependent transcriptional regulation of mtDNA- and nuclear-encoded mitochondria genes in the liver of newborn piglets." in: BMC veterinary research, Vol. 9, pp. 87, (2013) (PubMed).
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- 5-Hydroxymethylcytosine (5-hmC)
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别名
- 5-Hydroxymethylcytosine
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物质类
- Chemical
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背景
- In addition to this monoclonal, Active Motif offers two polyclonal antibodies that recognize 5-hydroxymethylcytosine, a whole serum version (39769) and a purified IgG version (39791).All are validated for use in methyl DNA immunoprecipitation (MeDIP). For customers that require the ability to quantitate the amount of IgG in the MeDIP reaction, we recommend either this monoclonal or the purified IgG polyclonal (39791). The whole serum version (39769) is very high titre and should be used carefully (0.1 - 0.5 µL per IP) as not to generate high non-specific background. The whole serum version (39769) has been used successfully in immunofluorescence (IF, Ito et al, 2010), and the purified IgG version (39791) is likely to work in this application as well. DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. 5-methylcytosine is a modified base that is found in the DNA of plants and vertebrates. A second type of DNA methylation exists, 5-hydroxymethylcytosine (5-hydroxy methylcytosine, 5-hmC). This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of cytosine oxygenases. This antibody was developed specifically to distinguish 5-hydroxymethylcytosine from 5-methylcytosine as conventional methods (enrichment by antibody or methyl DNA binding protein, enzymatic digestion and bisulfite sequencing) cannot do so. It is possible that 5-hydroxymethylcytosine (5-hmC) represents a pathway to demethylate DNA, as 5-hydroxymethylcytosine is repaired as mismatched DNA and replaced with unmethylated cytosine.
抗原
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