The stated application concentrations are suggested starting amounts. Titration of the MeCP2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,Flow Cytometry: 1:10-1:50
限制
仅限研究用
状态
Liquid
缓冲液
In 1X PBS, pH 7.4, with 0.09 % sodium azide
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Store at 4°C for up to one month. For long term, aliquot the MeCP2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
抗原
MECP2
(Methyl CpG Binding Protein 2 (MECP2))
别名
MeCP2
背景
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. [RefSeq].