CDC25B 抗体 (AA 119-248)
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- 抗原 See all CDC25B 抗体
- CDC25B (Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B))
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抗原表位
- AA 119-248
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CDC25B antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 纯化方法
- Antigen affinity
- 免疫原
- Amino acids 119-248 of human CDC25B were used as the immunogen for the CDC25B antibody.
- 亚型
- IgG
- Top Product
- Discover our top product CDC25B Primary Antibody
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anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (pSer186) antibody
CDC25B 适用: 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (pSer353) antibodyCDC25B 适用: 人, 小鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (pSer323) antibodyCDC25B 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (pSer323) antibodyCDC25B 适用: 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (pSer149) antibodyCDC25B 适用: 人, 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (AA 1-195) antibodyCDC25B 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (pSer353) antibodyCDC25B 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (AA 331-360) antibodyCDC25B 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB07936 unconjugated
anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (N-Term) antibodyCDC25B 适用: 人, 猴, 犬, 豚鼠, 兔 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) (pSer323) antibodyCDC25B 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Optimal dilution of the CDC25B antibody should be determined by the researcher.\. Western blot: 0.1-0.5 μg/mL,IHC (Paraffin): 0.5-1 μg/mL
- 限制
- 仅限研究用
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- 缓冲液
- 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
- 储存条件
- -20 °C
- 储存方法
- After reconstitution, the CDC25B antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
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- 抗原
- CDC25B (Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B))
- 别名
- CDC25B (CDC25B 产品)
- 背景
- Central to the onset of mitosis in all eukaryotic cells is the CDC2 protein kinase, the activity of which is negatively regulated by phosphorylation and positively activated by dephosphorylation. The latter function is carried out by a specific phosphatase, CDC25. At least 3 human CDC25 genes code for the A, B, and C forms of CDC25. CDC25B is mapped to 20p13. P38 kinase has a critical role in the initiation of a G2 delay after ultraviolet radiation. Inhibition of p38 blocks the rapid initiation of this checkpoint in both human and murine cells after ultraviolet radiation. In vitro, p38 binds and phosphorylates CDC25B at serines 309 and 361, and CDC25C at serine-216, phosphorylation of these residues is required for binding to 14-3-3 proteins. In vivo, inhibition of p38 prevents both phosphorylation of CDC25B at serine-309 and 14-3-3 binding after ultraviolet radiation, and mutation of this site is sufficient to inhibit the checkpoint initiation. Regulation of CDC25B phosphorylation by p38 is a critical event for initiating the G2/M checkpoint after ultraviolet radiation.
- UniProt
- P30305
- 途径
- Cell Division Cycle, M Phase, Autophagy
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