Recombinant Calponin 抗体
Quick Overview for Recombinant Calponin 抗体 (ABIN4949947)
抗原
抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯度
- Protein A affinity chromatography
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免疫原
- Recombinant protein was used as the immunogen for the Calponin antibody.
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亚型
- IgG kappa
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应用备注
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Optimal dilution of the Calponin antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min\. FACS: 0.5-1 μg/10^6 cells,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
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限制
- 仅限研究用
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浓度
- 0.2 mg/mL
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缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C,4 °C
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储存方法
- Store the Calponin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Calponin
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背景
- Calponin is a calmodulin, F-actin and tropomyosin binding protein which is thought to be involved in the regulation of smooth muscle contraction.
抗原
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