The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TIMP-2 antibody to be titered up or down for optimal performance.\. IHC (FFPE): 0.5-1 μg/mL for 30 min at RT,WB: 0.5-1 μg/mL,IF: 1-2 μg/mL
限制
仅限研究用
浓度
0.2 mg/mL
缓冲液
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C,-20 °C
储存方法
Store the TIMP-2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
抗原
TIMP2
(Metalloproteinase Inhibitor 2 (TIMP2))
别名
TIMP2
背景
It recognizes a protein of 21 kDa, identified as tissue inhibitor of metalloproteinases-2 (TIMP-2). It is closely related to TIMP-1 and shows the highest binding affinity to both the latent (pro) and active forms of 72 kDa Type IV collagenase (also known as MMP-2 or gelatinase A). It also has affinity for the active form of 92 kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.