Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)
纯化方法
Protein G affinity chromatography
免疫原
A partial recombinant protein from the N-terminal region of human Luteinizing hormone / Choriogonadotropin receptor was used as the immunogen for the hCG Receptor antibody.
Optimal dilution of the hCG Receptor antibody should be determined by the researcher.\. FACS: 0.5-1 μg/million cells in 0.1ml,IF: 1-2 μg/mL,WB: 0.5-1 μg/mL
限制
仅限研究用
浓度
1 mg/mL
缓冲液
1 mg/mL in 1X PBS, BSA free, sodium azide free
储存液
Azide free
储存条件
4 °C,-20 °C
储存方法
Store the hCG Receptor antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Recognizes a protein of 78-85 kDa, which is identified as the receptor for Luteinizing hormone / Choriogonadotropin. Luteinizing hormone plays a role in spermatogenesis and ovulation by stimulating the testes and ovaries to produce steroids. Choriogonadotropin production in the placenta maintains estrogen and progesterone levels during the first trimester of pregnancy. Ovaries and testes abundantly express luteinizing hormone / choriogonadotropin receptor (LHCGR) as a seven transmembrane, G protein-coupled receptor glycoprotein. LHCGR influences the protective effect of pregnancy and Gonadotropin against breast cancer. The expression of LHCGR on breast carcinoma correlates in part to the degree of tumor differentiation. LHCGR -positive breast tumors occur more frequently in tumors with greater cell differentiation in premenopausal women.