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EPO 抗体

This anti-EPO antibody is a 小鼠 单克隆 antibody detecting EPO in WB, FACS, IF 和 IHC (p). Suitable for 人.
产品编号 ABIN4949655
发货至: 中国

Quick Overview for EPO 抗体 (ABIN4949655)

抗原

See all EPO 抗体
EPO (Erythropoietin (EPO))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This EPO antibody is un-conjugated

应用范围

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Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

EPO-1368
  • 纯化方法

    Protein G affinity chromatography

    免疫原

    A human partial recombinant protein was used as the immunogen for the EPO antibody.

    亚型

    IgG
  • 应用备注

    Titering of the EPO antibody may be required for optimal performance.

    1. Required HIER: boil tissue sections in 10  mM citrate buffer,  pH 6, for 10-20 min.\. WB: 0.5-1 μg/mL,IF: 1-2 μg/mL,Immunohistochemistry (FFPE): 4-8 μg/mL for 30 min at RT (1),Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

    限制

    仅限研究用
  • 浓度

    1 mg/mL

    缓冲液

    1 mg/mL in 1X PBS, BSA free, sodium azide free

    储存液

    Azide free

    储存条件

    4 °C,-20 °C

    储存方法

    Store the EPO antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原

    EPO (Erythropoietin (EPO))

    别名

    Erythropoietin / EPO

    物质类

    Hormone

    背景

    Erythropoietin / EPO is a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. It is the primary factor responsible for regulating erythropoiesis during steady-state conditions and in response to blood loss and hemorrhage in the adult organism. Erythropoietin is synthesized by the kidney and stimulates the proliferation and maturation of bone marrow erythroid precursor cells. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis.

    途径

    JAK/STAT Signaling, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity
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