Optimal dilution of the Connexin 32 antibody should be determined by the researcher.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,WB: 0.5-1 μg/mL,IHC (Frozen): 0.5-1 μg/mL for 30 min at RT
限制
仅限研究用
浓度
1 mg/mL
缓冲液
1 mg/mL in 1X PBS, BSA free, sodium azide free
储存液
Azide free
储存条件
4 °C,-20 °C
储存方法
Store the Connexin 32 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
This Ab recognizes a protein of 27-32 kDa, identified as Connexin 32. The connexin family of proteins forms hexameric complexes called connexons that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane alpha-helical domains, two extracellular loops, a cytoplasmic loop and cytoplasmic N- and C-termini. Many of the key functional differences arise from specific amino-acid substitutions in the most highly conserved domains, the transmembrane and extracellular regions. Each of the approximately 20-connexin isoforms produces channels with distinct permeability and electrical and chemical sensitivities, therefore, one connexin usually cannot fully substitute for another.