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CD35 抗体

This anti-CD35 antibody is a 小鼠 单克隆 antibody detecting CD35 in FACS, IF 和 IHC (p). Suitable for 人, Baboon 和 猴.
产品编号 ABIN4949510
发货至: 中国

Quick Overview for CD35 抗体 (ABIN4949510)

抗原

See all CD35 (CR1) 抗体
CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

适用

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人, Baboon, 猴

宿主

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小鼠

克隆类型

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单克隆

标记

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This CD35 antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

CR1-802
  • 纯化方法

    Protein G affinity chromatography

    免疫原

    Recombinant human protein was used as the immunogen for the CD35 antibody.

    亚型

    IgG1 kappa
  • 应用备注

    Optimal dilution of the CD35 antibody should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

    限制

    仅限研究用
  • 浓度

    1 mg/mL

    缓冲液

    1 mg/mL in 1X PBS, BSA free, sodium azide free

    储存液

    Azide free

    储存条件

    4 °C,-20 °C

    储存方法

    Store the CD35 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原

    CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

    别名

    CD35

    背景

    Recognizes a protein of 210-220  kDa, which is identified as the complement receptor 1 (CR1)/CD35. This mAb does not block CR1 activity. It is highly specific to CR1 and shows no cross-reaction with CR2. The primary function of CR1 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. The Knops blood group system is a system of antigens located on this protein. Follicular dendritic cells (FDC) are restricted to the B-cell regions of secondary lymphoid follicles. They are CD21+/CD35+/CD1a-. This mAb labels follicular dendritic cells and follicular dendritic cell sarcoma.

    途径

    Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Endopeptidase Activity
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