Elastase 3B 抗体 (AA 82-238)
Quick Overview for Elastase 3B 抗体 (AA 82-238) (ABIN4949400)
抗原
See all Elastase 3B (ELA3B) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 82-238
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纯化方法
- Protein G
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免疫原
- A partial recombinant protein (aa 82-238) was used as the immunogen for the Elastase 3B antibody.
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亚型
- IgG1
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应用备注
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Optimal dilution of the Elastase 3B antibody should be determined by the researcher.
1. FFPE staining requires\. ELISA: 2-4 μg/mL, order BSA free format for coating,FACS: 0.5-1 μg/10^6 cells in 0.1ml,IF: 1-2 μg/mL, IHC-P: 1-2 μg/mL for 30 min at RT
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the Elastase 3B antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))
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别名
- CELA3B / Elastase 3B
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背景
- Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six genes which encode the structurally similar proteins: Elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, 3B has little elastolytic activity. Like most of the human elastases, 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. It may also function in the intestinal transport and metabolism of cholesterol. Elastases 3A/B have been referred to as Protease E and as Elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays.
抗原
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