Recombinant CD99 抗体
Quick Overview for Recombinant CD99 抗体 (ABIN4949314)
抗原
See all CD99 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Protein A affinity chromatography
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免疫原
- Human protein was used as the immunogen for the recombinant CD99 antibody.
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亚型
- IgG kappa
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应用备注
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Titering of the recombinant CD99 antibody may be required for optimal performance.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 2-4 μg/million cells in 0.1ml,Immunofluorescence: 2-4 μg/mL,Immunohistochemistry (FFPE): 5-10 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the recombinant CD99 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- CD99
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别名
- CD99
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背景
- CD99 is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, it may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. Cyclophilin A binds to CD99 and may act as a signaling regulator of the protein. [Wiki]
抗原
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