Recombinant CD34 抗体
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北京 101111
Quick Overview for Recombinant CD34 抗体 (ABIN4949293)
抗原
See all CD34 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Protein A affinity chromatography
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免疫原
- Recombinant full-length human protein was used as the immunogen for the recombinant CD34 antibody.
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亚型
- IgG kappa
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anti-CD34 (CD34) (AA 366-382), (C-Term) antibody
CD34 适用: 人, 大鼠, 小鼠 FACS, WB, IHC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-CD34 (CD34) (AA 201-300) antibodyCD34 适用: 人, 大鼠, 小鼠, 兔 FACS, WB, ELISA, IHC (p), IHC (fro), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-CD34 (CD34) (AA 301-385) antibodyCD34 适用: 人, 大鼠, 小鼠, 兔, 小鸡, 山羊 FACS, WB, ELISA, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-CD34 (CD34) (AA 36-287) antibodyCD34 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD34 (CD34) (C-Term) antibodyCD34 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD34 (CD34) antibody (PE)CD34 适用: 人 FACS, ICC 宿主: 小鼠 Monoclonal 4H11[APG] PE
anti-CD34 (CD34) antibodyCD34 适用: 人, Non-Human Primate FACS, WB, IP, IHC (p), IHC (fro), Func 宿主: 小鼠 Monoclonal QBEnd-10 unconjugated
anti-CD34 (CD34) antibodyCD34 适用: 人, 大鼠 FACS, WB, IHC 宿主: 兔 Monoclonal HPCA1-2598R unconjugated Recombinant Antibody
anti-CD34 (CD34) (AA 151-385) antibodyCD34 适用: 人, 大鼠 WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-CD34 (CD34) (AA 124-375) antibodyCD34 适用: 人 WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Optimal dilution of the recombinant CD34 antibody should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 2-4 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 2-4 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the recombinant CD34 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- CD34
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别名
- CD34
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背景
- This antibody recognizes a transmembrane, heavily glycosylated protein of 90-120 kDa, which is identified as CD34. Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels > 85 % of angiosarcoma and Kaposi s sarcoma, but shows low specificity.
抗原
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