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- 抗原
- CD20 (B Cells)
- 适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- 非结合性
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 纯化方法
- Protein A affinity chromatography
- 免疫原
- Recombinant human CD20 protein was used as the immunogen for this CD20 antibody.
- 亚型
- IgG
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anti-CD20 (B Cells) antibody
适用: 人 FACS, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Titering of the CD20 antibody may be required for optimal performance.\. FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT
- 限制
- 仅限研究用
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- 浓度
- 0.2 mg/mL
- 缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- CD20 antibody with azide can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
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- 抗原
- CD20 (B Cells)
- 别名
- CD20 (B-cell marker)
- 背景
- CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. This antibody can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin s disease, particularly of lymphocyte predominant type.
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