CD20 (B Cells) (Extracellular Domain) 抗体
适用: 人
FACS, IF, IHC (fro)
宿主: 小鼠
Monoclonal
B342 (B9E9)
unconjugated
(2 references)-
- 抗原
- CD20 (B Cells)
- 抗原表位
- Extracellular Domain
- 适用
- 人
-
宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- This antibody reacts with an antigen present on most of the B-cells. They react with a 33 kD polypeptide and a minor component of a 30 kD polypeptide present in B-cells.
- 纯化方法
- Purified.
- 免疫原
- Lymphoblastoid cell line DAUDI.
- 克隆位点
- B342 (B9E9)
- 亚型
- IgG2a
-
anti-CD20 (B Cells) antibody
适用: 人 FACS, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-CD20 (B Cells) antibody适用: 人 FACS, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Immunofluorescence: 5-20 ug/mL
Flow cytometry: 1-5 ug/10^6 cells
Western blotting: 1-5 ug/mL.
Immunohistochemistry: 5-20 mg/mL on frozen sections using avidin-biotin system. This antibody was not quality controlled in flow cytometry.
Staining Procedure: This antibody can be used on frozen cryostat sections. - 说明
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Cellular Localization: Cell membrane.
Recommended Positive Control: Tonsil and Lymph Node - 限制
- 仅限研究用
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- 浓度
- 0.2 mg/ml.
- 缓冲液
- 50 mM Sodium phosphate, 150 mM Sodium Chloride, BSA 0.2%, pH 8.0
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
-
-
: "Expression of calcium-permeable cation channel CD20 accelerates progression through the G1 phase in Balb/c 3T3 cells." in: The Journal of biological chemistry, Vol. 270, Issue 22, pp. 13099-104, (1995) (PubMed).
: "Transfection of the CD20 cell surface molecule into ectopic cell types generates a Ca2+ conductance found constitutively in B lymphocytes." in: The Journal of cell biology, Vol. 121, Issue 5, pp. 1121-32, (1993) (PubMed).
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: "Expression of calcium-permeable cation channel CD20 accelerates progression through the G1 phase in Balb/c 3T3 cells." in: The Journal of biological chemistry, Vol. 270, Issue 22, pp. 13099-104, (1995) (PubMed).
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- 抗原
- CD20 (B Cells)
- 分子量
- 33-37 kDa
- 基因ID
- 931
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