Recombinant Keratin Basic 抗体
Quick Overview for Recombinant Keratin Basic 抗体 (ABIN4949251)
抗原
See all Keratin Basic 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Protein A affinity chromatography
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免疫原
- Recombinant human KRT76 protein was used as the immunogen for the recombinant Basic Cytokeratin antibody.
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亚型
- IgG kappa
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anti-Keratin Basic antibody
适用: 人, 大鼠 WB, IF, FACS, IHC (p), ELISA 宿主: 小鼠 Monoclonal KRTH-1076 unconjugated
anti-Keratin Basic antibody适用: 人, 大鼠, 小鼠 WB, IF, FACS, IHC, StM 宿主: 小鼠 Monoclonal SPM591 unconjugated
anti-Keratin Basic antibody适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Monoclonal KRTH-1576R unconjugated Recombinant Antibody
anti-Keratin Basic antibody适用: 人, 大鼠, 小鼠, 兔, Cow, 小鸡, 猴, 犬 WB, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal AE3 unconjugated
anti-Keratin Basic antibody适用: 人, 大鼠, 小鼠, 兔, Cow, 小鸡, 猴, 犬 WB, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal AE3 unconjugated
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应用备注
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Optimal dilution of the recombinant Basic Cytokeratin antibody should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the recombinant Basic Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Keratin Basic
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别名
- Basic Cytokeratin
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背景
- This mAb recognizes basic (Type II or HMW) cytokeratins, which include 67 kDa (CK1), 64 kDa (CK3), 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 52 kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40 kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
抗原
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