Keratin Basic 抗体
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- 抗原 See all Keratin Basic 抗体
- Keratin Basic
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适用
- 人, 大鼠, 小鼠, 兔, Cow, 小鸡, 猴, 犬
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 纯化方法
- Protein G purified
- 免疫原
- Solubilized cytokeratin extract from human stratum corneum was used as the immunogen.
- 克隆位点
- AE3
- 亚型
- IgG1
- Top Product
- Discover our top product Primary Antibody
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anti-Keratin Basic antibody
适用: 人, 大鼠 WB, IF, FACS, IHC (p), ELISA 宿主: 小鼠 Monoclonal KRTH-1076 unconjugated
anti-Keratin Basic antibody适用: 人, 大鼠, 小鼠 WB, IF, FACS, IHC, StM 宿主: 小鼠 Monoclonal SPM591 unconjugated
anti-Keratin Basic antibody适用: 人, 大鼠, 小鼠, 兔, Cow, 小鸡, Non-Human Primate WB, IHC (p), IHC (fro) 宿主: 小鼠 Monoclonal AE3 unconjugated
anti-Keratin Basic antibody适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Monoclonal KRTH-1576R unconjugated Recombinant Antibody
anti-Keratin Basic antibody适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Monoclonal KRTH-1576R unconjugated Recombinant Antibody
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- 应用备注
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Differences in protocols and secondaries may cause the Basic Cytokeratin antibody to need titering for optimal performance.
1. FFPE staining requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,WB: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2) - 限制
- 仅限研究用
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- 浓度
- 0.2 mg/mL
- 缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store the Basic Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- 抗原
- Keratin Basic
- 别名
- Basic Cytokeratin (Keratin Basic 产品)
- 背景
- Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. This antibody recognizes basic (Type II or HMW) cytokeratins, which include 67 kDa (CK1), 64 kDa (CK3), 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 52 kDa (CK8). Many studies have shown the usefulness of keratin markers in cancer research and tumor diagnosis. The basic family clone AE3 is commonly used in combination with clone AE1 as a pan cytokeratin antibody acidic + basic cocktail (< a href=../pan-cytokeratin-antibody-cocktail-ae1-ae3-v2330>Cat No V2330).
- 基因ID
- 3848
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