RICTOR 抗体
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北京 101111
Quick Overview for RICTOR 抗体 (ABIN492626)
抗原
See all RICTOR 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Recognizes Rictor.
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产品特性
- Synonyms: KIAA1999, Rapamycin-insensitive companion of mTOR, mAVO3
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纯化方法
- Subsequent Ultrafiltration and Size Exclusion Chromatography.
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组件
- incl. positive Control
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免疫原
- Peptide conjugated to hemocyanin derived from Rictor Sequence.
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亚型
- IgG1
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anti-RPTOR Independent Companion of mTOR, Complex 2 (RICTOR) (AA 1-98) antibody
RICTOR 适用: 人 WB, ELISA, IHC (p), IP 宿主: 小鼠 Monoclonal 1F3 unconjugated
anti-RPTOR Independent Companion of mTOR, Complex 2 (RICTOR) antibodyRICTOR 适用: 人, 小鼠, 猴 WB, IHC, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 7B3 unconjugated
anti-RPTOR Independent Companion of mTOR, Complex 2 (RICTOR) (C-Term) antibodyRICTOR 适用: 人 WB, IHC, IHC (p), IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-RPTOR Independent Companion of mTOR, Complex 2 (RICTOR) (AA 23-264) antibodyRICTOR 适用: 人 WB, IHC, ELISA, IF, FACS, ICC 宿主: 兔 Polyclonal unconjugated
anti-RPTOR Independent Companion of mTOR, Complex 2 (RICTOR) antibodyRICTOR 适用: 人, 小鼠 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-RPTOR Independent Companion of mTOR, Complex 2 (RICTOR) (AA 1-265) antibodyRICTOR 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-RPTOR Independent Companion of mTOR, Complex 2 (RICTOR) (AA 1-245) antibodyRICTOR 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-RPTOR Independent Companion of mTOR, Complex 2 (RICTOR) (AA 1-99) antibodyRICTOR 适用: 人 WB, IHC, ELISA, IHC (p), IP 宿主: 小鼠 Monoclonal 1F3 unconjugated
anti-RPTOR Independent Companion of mTOR, Complex 2 (RICTOR) (C-Term) antibodyRICTOR 适用: 人 WB, IHC, IP 宿主: 兔 Monoclonal BLR370N unconjugated Recombinant Antibody
anti-RPTOR Independent Companion of mTOR, Complex 2 (RICTOR) (C-Term) antibodyRICTOR 适用: 人 WB, IHC, IP 宿主: 兔 Monoclonal BLR370N unconjugated Recombinant Antibody
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应用备注
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Immunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer AS00002BU-N or AS00002BU-L. Included Positive Control: Cell lysate from untreated HeLa cells (See Protocols for moredetails).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
实验流程
- Positive Control Provided. Cell lysate from untreated Hela cellsDescription: Cell lysate from untreated HeLa cells, epitheloid cervical carcinoma (Human)Format: Lyophilized cell lysate from serum starved HeLa cells. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles.
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限制
- 仅限研究用
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溶解方式
- Restore with 1 mL H2O (15 min, RT).
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缓冲液
- 1 mLPBS, containing 0.09 % Sodium Azide/PEG and Sucrose.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -80 °C up to 1 year. Avoid repeated freeze / thaw cycles. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.
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有效期
- 12 months
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- RICTOR (RPTOR Independent Companion of mTOR, Complex 2 (RICTOR))
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别名
- RICTOR
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背景
- The mTOR pathway (mammalian target of rapamycin) coordinates nutrient signals with growth factor dependent signaling. Recent data revealed that mTOR is organized in two independent protein complexes: The complex containing mTOR, GbL and RAPTOR is target of rapamycin while the complex containing mTOR, GbL and RICTOR is insensitive to rapamycin. The mTOR /RICTOR complex phosphorylates PKBalpha/akt1 at Serine 473Synonyms: KIAA1999, Rapamycin-insensitive companion of mTOR, mAVO3
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基因ID
- 253260
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UniProt
- Q6R327
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途径
- Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, CXCR4-mediated Signaling Events
抗原
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