GSK3 alpha 抗体 (pTyr215, pTyr278)
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Quick Overview for GSK3 alpha 抗体 (pTyr215, pTyr278) (ABIN492620)
抗原
See all GSK3 alpha (GSK3a) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- pTyr215, pTyr278
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特异性
- This antibody specifically recognizes GSK3beta phosphorylated at Tyr215 at 46 kDa and GSK3alpha phosphorylated at Tyr278 at 54 kDa in Western blot applications.
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产品特性
- Synonyms: Glycogen synthase kinase-3 alpha, GSK3A, GSK-3 alpha, Factor A
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纯化方法
- Subsequent Ultrafiltration and Size Exclusion Chromatography.
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组件
- incl. positive Control
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免疫原
- Phosphopeptide conjugated to hemocyaninEpitope: Phosphotyrosine 215, Phosphotyrosine 278AA Sequence: N V S pY I C S
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亚型
- IgG1
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anti-Glycogen Synthase Kinase 3 alpha (GSK3a) (pSer21) antibody
GSK3a 适用: 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Glycogen Synthase Kinase 3 alpha (GSK3a) antibodyKO Validated GSK3a 适用: 人 WB, IP 宿主: 兔 Monoclonal unconjugated
anti-Glycogen Synthase Kinase 3 alpha (GSK3a) (pTyr216), (pTyr279) antibodyGSK3a 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Glycogen Synthase Kinase 3 alpha (GSK3a) (pSer21) antibodyGSK3a 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Glycogen Synthase Kinase 3 alpha (GSK3a) (AA 122-406) antibodyGSK3a 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glycogen Synthase Kinase 3 alpha (GSK3a) (AA 409-483) antibodyGSK3a 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Glycogen Synthase Kinase 3 alpha (GSK3a) (AA 76-105), (N-Term) antibodyGSK3a 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB3817-3818 unconjugated
anti-Glycogen Synthase Kinase 3 alpha (GSK3a) antibodyKD Validated GSK3a 适用: 人 WB, FACS 宿主: 兔 Monoclonal 23GB855 unconjugated Recombinant Antibody
anti-Glycogen Synthase Kinase 3 alpha (GSK3a) antibodyGSK3a 适用: 人 WB, ELISA, ICC 宿主: 小鼠 Monoclonal 9D5G1 unconjugated
anti-Glycogen Synthase Kinase 3 alpha (GSK3a) antibodyGSK3a 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 6G2F1 unconjugated
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应用备注
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Immunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer AS00002BU-N or AS00002BU-L. Included Positive Control: Cell lysate from pervanadate treated HepG2 cells (See Protocolsfor more details).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
实验流程
- Positive Control Provided. Cell lysate from pervanadate-treated HepG2 cellsDescription: Cell lysate from pervanadate-treated HepG2 cells, hepatocellular carcinoma(Human)Format: Lyophilized cell lysate from from HepG2 cells. Serum starved cells were treated for 15min with pervanadate. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles.
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限制
- 仅限研究用
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溶解方式
- Restore with 1 mL H2O (15 min, RT).
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缓冲液
- 1 mLPBS with 0.09 % Sodium Azide/PEG and Sucrose
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C/-80 °C
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储存方法
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Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch. -
有效期
- 12 months
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- GSK3 alpha (GSK3a) (Glycogen Synthase Kinase 3 alpha (GSK3a))
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别名
- GSK3 alpha
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背景
- Glycogen Synthase Kinase 3 beta (GSK3beta) is a serine/threonine kinase that is acting independently of most signal transduction pathways. The activity of GSK3beta can be modulated by phosphorylation of Ser9 and Tyr215 in the activation loop. GSK3beta plays a crucial role in phosphorylation of beta-catenin and thus is an important kinase involved in the regulation of beta-catenin/wnt-signaling.Synonyms: Factor A, GSK-3 alpha, GSK3A, Glycogen synthase kinase-3 alpha
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基因ID
- 2931
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UniProt
- P49840
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途径
- PI3K-Akt Signaling, WNT signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, BCR Signaling, Warburg Effect
抗原
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