GSK3 alpha 抗体 (pTyr215, pTyr278) (Biotin)
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- 抗原 See all GSK3 alpha (GSK3a) 抗体
- GSK3 alpha (GSK3a) (Glycogen Synthase Kinase 3 alpha (GSK3a))
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抗原表位
- pTyr215, pTyr278
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适用
- 人, 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This GSK3 alpha antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB)
- 特异性
- This antibody specifically recognizes GSK3beta phosphorylated at Tyr215 at 46 kDa and GSK3alpha phosphorylated at Tyr278 at 54 kDa in Western blot applications.
- 产品特性
- Synonyms: Glycogen synthase kinase-3 alpha, GSK3A, GSK-3 alpha, Factor A
- 纯化方法
- Subsequent Ultrafiltration and Size Exclusion Chromatography.
- 组件
- incl. positive Control
- 免疫原
- Phosphopeptide conjugated to hemocyaninEpitope: Phosphotyrosine 215, Phosphotyrosine 278AA Sequence: N V S pY I C S
- 克隆位点
- 6D3
- 亚型
- IgG1
- Top Product
- Discover our top product GSK3a Primary Antibody
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anti-Glycogen Synthase Kinase 3 alpha (GSK3a) (pSer21) antibody
GSK3a 适用: 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Glycogen Synthase Kinase 3 alpha (GSK3a) (pSer21) antibodyGSK3a 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Glycogen Synthase Kinase 3 alpha (GSK3a) antibodyKO Validated GSK3a 适用: 人 WB, IP 宿主: 兔 Monoclonal unconjugated
anti-Glycogen Synthase Kinase 3 alpha (GSK3a) (AA 122-406) antibodyGSK3a 适用: 小鼠 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glycogen Synthase Kinase 3 alpha (GSK3a) (AA 76-105), (N-Term) antibodyGSK3a 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB3817-3818 unconjugated
anti-Glycogen Synthase Kinase 3 alpha (GSK3a) (AA 409-483) antibodyGSK3a 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Glycogen Synthase Kinase 3 alpha (GSK3a) antibodyGSK3a 适用: 人 WB, ELISA, ICC 宿主: 小鼠 Monoclonal 9D5G1 unconjugated
anti-Glycogen Synthase Kinase 3 alpha (GSK3a) antibodyGSK3a 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 6G2F1 unconjugated
anti-Glycogen Synthase Kinase 3 alpha (GSK3a) (Ser21) antibodyGSK3a 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Glycogen Synthase Kinase 3 alpha (GSK3a) (AA 36-118) antibodyGSK3a 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Immunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer AS00002BU-N or AS00002BU-L. Included Positive Control: Cell lysate from pervanadate treated HepG2 cells (See Protocolsfor more details).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- Positive Control Provided. Cell lysate from pervanadate-treated HepG2 cellsDescription: Cell lysate from pervanadate-treated HepG2 cells, hepatocellular carcinoma(Human)Format: Lyophilized cell lysate from from HepG2 cells. Serum starved cells were treated for 15min with pervanadate. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles.
- 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- PBS with 0.09 % Sodium Azide/PEG and Sucrose.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
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Aliquote and freeze in liquid nitrogen at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch. - 有效期
- 12 months
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- 抗原
- GSK3 alpha (GSK3a) (Glycogen Synthase Kinase 3 alpha (GSK3a))
- 别名
- GSK3 alpha (GSK3a 产品)
- 别名
- 2700086H06Rik antibody, GSK3A antibody, GSK-3[a] antibody, GSK-3[b] antibody, GSK3 antibody, gsk3a antibody, xgsk3 antibody, glycogen synthase kinase 3 alpha antibody, glycogen synthase kinase 3 alpha b antibody, glycogen synthase kinase 3 alpha S homeolog antibody, GSK3A antibody, Gsk3a antibody, gsk3a antibody, gsk3ab antibody, gsk3a.S antibody, Bm1_31380 antibody
- 背景
- Glycogen Synthase Kinase 3 beta (GSK3beta) is a serine/threonine kinase that is acting independently of most signal transduction pathways. The activity of GSK3beta can be modulated by phosphorylation of Ser9 and Tyr215 in the activation loop. GSK3beta plays a crucial role in phosphorylation of beta-catenin and thus is an important kinase involved in the regulation of beta-catenin/wnt-signaling.Synonyms: Factor A, GSK-3 alpha, GSK3A, Glycogen synthase kinase-3 alpha
- 基因ID
- 2931
- UniProt
- P49840
- 途径
- PI3K-Akt Signaling, WNT signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, BCR Signaling, Warburg Effect
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