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LC3B 抗体 (N-Term)

Name: LC3B 抗体 (N-Term)
SKU: ABIN492615
Availability: OutOfStock

This anti-LC3B antibody is a 小鼠单克隆 antibody detecting LC3B in WB 和 IF. Suitable for 人, 小鼠, 大鼠, Hamster 和猴.

Western Blotting (WB) image for anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (N-Term) antibody (ABIN492615) (LC3B 抗体 (N-Term))

1 image

产品编号 ABIN492615

发货至: 中国

Quick Overview for LC3B 抗体 (N-Term) (ABIN492615)

抗原

See all LC3B (MAP1LC3B) 抗体

LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

适用

人, 小鼠, 大鼠, Hamster, 猴

宿主

小鼠

克隆类型

单克隆

标记

This LC3B antibody is un-conjugated

应用范围

Western Blotting (WB), Immunofluorescence (IF)

克隆位点

2G6

Discover our top product MAP1LC3B Primary Antibody

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N-Term

特异性

This antibody specifically recognizes both forms of endogenous LC3, the cytoplasmic LC3-I (18 kDa) as well as the lipidated form generated during autophagosome and autophagolysosome formation: LC3-II (16 kDa).Aff. Const.:Human, Mouse, Rat, Monkey and Hamster.

产品特性

Synonyms: MAP1LC3B, MAP1A/MAP1B, Map1lc3b, Map1alc3, Map1lc3, Microtubule-associated protein1 light chain 3 beta, Microtubule-associated proteins 1A/1B light chain 3B,Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1A /1B light chain 3 B

纯化方法

Subsequent Ultrafiltration and Size Exclusion Chromatography.

组件

incl. positive Control

免疫原

Synthetic peptide hemocyanin conjugated derived from the N-terminus of LC3-B

亚型

IgG1
anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) antibody
MAP1LC3B 适用: 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) antibody
KO Validated MAP1LC3B 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated

anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) antibody
MAP1LC3B 适用: 人, 小鼠, 大鼠 WB, IHC (p), FACS 宿主: 兔 Polyclonal unconjugated

anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) antibody
MAP1LC3B 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 5H12 unconjugated

anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 74-106) antibody
MAP1LC3B 适用: 人, 小鼠 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated

anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 1-30), (N-Term) antibody
MAP1LC3B 适用: 人, 大鼠 WB, IF, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated

anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (N-Term) antibody
MAP1LC3B 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) antibody
MAP1LC3B 适用: 人 WB, IF 宿主: 小鼠 Monoclonal unconjugated

anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 89-122), (cleaved) antibody
MAP1LC3B 适用: 人, 小鼠 IF, ICC 宿主: 兔 Polyclonal RB15839-RB28608 unconjugated

anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 77-106) antibody
MAP1LC3B 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

应用备注

Immunoblotting: 0.5 μg/mL for HRPO/ECL detectionRecommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. We strongly recommend to use PVDF membranes for immunoblot analysis. Immunocytochemistry: Use at 1-10 μg/mL (Paraformaldehyd/Methanol fixation). Included Positive Control: Cell lysate from untreated Neuro 2A (See Protocols).

实验流程

Positive Control: Cell lysate from untreated Neuro 2A cells, brain endothelioma (Mouse)Format: Lyophilized cell lysate from serum starved Neuro 2A. Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20. 000 cells. Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended forapplications with native proteins such as in immunoprecipitation.

限制

仅限研究用
浓度

0.1 mg/mL

缓冲液

PBS containing 0.09 % Sodium Azide, PEG and Sucrose/50 % Glycerol

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store the antibody (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

有效期

12 months
抗原

LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

别名

LC3B

背景

Autophagy is an alternative process of proteasomal degradation for some long-lived proteins or organelles. Alterations in the autophagic-lysosomal compartment have been linked to neuronal death in many neurodegenerative disorders as well as in transmissible neuronal pathologies (prion diseases). Genetic studies in yeast have shown that Autophagy-defective Gene-8 (Atg-8) represents a specific marker for autophagy. Among the four families of mammalian Atg8-related proteins only LC3 (microtubule-associated protein1 light chain 3) is expressed at sufficient high levels and efficiently recruited to autophagic vesicles in cells and tissues. During autophagy the cytoplasmic form, LC3-I is processed and recruited to autophagosomes, where LC3-II is generated by site specific proteolysis near to the C-terminus. Autophagic vacuoles have been also reported frequently in cardiomyopathies or muscle cells exposed to different experimental settings.Synonyms: Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1A / 1B light chain 3 B, MAP1A/MAP1B, MAP1LC3B, Map1alc3, Map1lc3, Map1lc3b, Microtubule-associated protein 1 light chain 3 beta, Microtubule-associated proteins 1A/1B light chain 3B

基因ID

81631

UniProt

Q9GZQ8

途径

Autophagy