LC3B 抗体 (N-Term)
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- 抗原 See all LC3B (MAP1LC3B) 抗体
- LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))
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抗原表位
- N-Term
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适用
- 人, 小鼠, 大鼠, Hamster, 犬
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This LC3B antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF)
- 特异性
- This antibody specifically recognizes both forms of endogenous LC3, the cytoplasmic LC3-I (18 kDa) as well as the lipidated form generated during autophagosome and autophagolysosome formation: LC3-II (16 kDa). Immunocytochemical staining of cells with LC3 antibody Cat.-No AM20212PU-N (Clone 5F10) reveals the specific punctate distribution of endogenous LC3-II as a hallmark of autophagic activity.
- 产品特性
- Synonyms: MAP1LC3B, MAP1A/MAP1B, Map1lc3b, Map1alc3, Map1lc3, Microtubule-associated protein1 light chain 3 beta, Microtubule-associated proteins 1A/1B light chain 3B,Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1A /1B light chain 3 B
- 纯化方法
- Subsequent Ultrafiltration and Size Exclusion Chromatography
- 组件
- incl. positive Control
- 免疫原
- Synthetic peptide hemocyanin conjugated derived from the N-terminus of LC3-B
- 克隆位点
- 5F10
- 亚型
- IgG1
- Top Product
- Discover our top product MAP1LC3B Primary Antibody
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anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) antibody
MAP1LC3B 适用: 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 1-50) antibodyMAP1LC3B 适用: 人, 小鼠 WB, IHC, IF, FACS, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) antibodyKO Validated MAP1LC3B 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated
anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) antibodyMAP1LC3B 适用: 人, 小鼠, 大鼠 WB, IHC (p), FACS 宿主: 兔 Polyclonal unconjugated
anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 1-100) antibodyMAP1LC3B 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) antibodyMAP1LC3B 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 5H12 unconjugated
anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 1-30), (N-Term) antibodyMAP1LC3B 适用: 人, 大鼠 WB, IF, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 74-106) antibodyMAP1LC3B 适用: 人, 小鼠 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (N-Term) antibodyMAP1LC3B 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 89-122), (cleaved) antibodyMAP1LC3B 适用: 人, 小鼠 IF, ICC 宿主: 兔 Polyclonal RB15839-RB28608 unconjugated
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- 应用备注
- Immunoblotting: 0.5 μg/mL for HRPO/ECL detectionRecommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. We strongly recommend to use PVDF membranes for immunoblot analysis. Immunocytochemistry: Use at 1-10 μg/mL (Paraformaldehyd/Methanol fixation). Included Positive Control: Cell lysate from untreated Neuro 2A (See Protocols).
- 实验流程
- Positive Control: Cell lysate from untreated Neuro 2A cells, brain endothelioma (Mouse)Format: Lyophilized cell lysate from serum starved Neuro 2A. Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20. 000 cells. Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended forapplications with native proteins such as in immunoprecipitation.
- 限制
- 仅限研究用
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- 溶解方式
- Restore with 1 mL H2O (15 min, RT)
- 缓冲液
- 1 mLPBS, 0.09 % Sodium Azide, PEG and Sucrose
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C/-80 °C
- 储存方法
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Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. - 有效期
- 12 months
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- 抗原
- LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))
- 别名
- LC3B (MAP1LC3B 产品)
- 别名
- ATG8F antibody, LC3B antibody, MAP1A/1BLC3 antibody, MAP1LC3B-a antibody, Map1lc3 antibody, Mpl3 antibody, zbs559 antibody, 1010001C15Rik antibody, Atg8 antibody, LC3b antibody, zgc:56434 antibody, wu:fb60g11 antibody, map1lc3b antibody, MGC76283 antibody, MAP1LC3B antibody, microtubule associated protein 1 light chain 3 beta antibody, microtubule-associated protein 1 light chain 3 beta antibody, microtubule associated protein 1 light chain 3 beta L homeolog antibody, MAP1LC3B antibody, Map1lc3b antibody, map1lc3b antibody, map1lc3b.L antibody
- 背景
- Autophagy is an alternative process of proteasomal degradation for some long-lived proteins or organelles. Alterations in the autophagic-lysosomal compartment have been linked to neuronal death in many neurodegenerative disorders as well as in transmissible neuronal pathologies (prion diseases). Genetic studies in yeast have shown that Autophagy-defective Gene-8 (Atg-8) represents a specific marker for autophagy. Among the four families of mammalian Atg8-related proteins only LC3 (microtubule-associated protein1 light chain 3) is expressed at sufficient high levels and efficiently recruited to autophagic vesicles in cells and tissues. During autophagy the cytoplasmic form, LC3-I is processed and recruited to autophagosomes, where LC3-II is generated by site specific proteolysis near to the C-terminus. Autophagic vacuoles have been also reported frequently in cardiomyopathies or muscle cells exposed to different experimental settings.Synonyms: Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1A / 1B light chain 3 B, MAP1A/MAP1B, MAP1LC3B, Map1alc3, Map1lc3, Map1lc3b, Microtubule-associated protein 1 light chain 3 beta, Microtubule-associated proteins 1A/1B light chain 3B
- 分子量
- 18 kDa (LC3-I), 16 kDa (LC-II)
- 基因ID
- 81631
- UniProt
- Q9GZQ8
- 途径
- Autophagy
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