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N-Cadherin 抗体 (Cytoplasmic Domain) (Biotin)

CDH2 适用: 人, 小鼠 WB 宿主: 小鼠 Monoclonal 7-00E-009 Biotin
产品编号 ABIN492339
发货至: 中国
  • 抗原 See all N-Cadherin (CDH2) 抗体
    N-Cadherin (CDH2) (Cadherin 2 (CDH2))
    抗原表位
    • 22
    • 15
    • 14
    • 9
    • 6
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
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    • 2
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    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cytoplasmic Domain
    适用
    • 130
    • 93
    • 65
    • 21
    • 16
    • 14
    • 8
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    人, 小鼠
    宿主
    • 85
    • 54
    • 4
    • 4
    • 3
    小鼠
    克隆类型
    • 78
    • 72
    单克隆
    标记
    • 84
    • 16
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
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    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This N-Cadherin antibody is conjugated to Biotin
    应用范围
    • 116
    • 58
    • 42
    • 39
    • 27
    • 22
    • 17
    • 14
    • 14
    • 13
    • 12
    • 8
    • 4
    • 2
    • 1
    • 1
    Western Blotting (WB)
    特异性
    This antibody specifically recognizes N-Cadherin at 127 kDa.
    产品特性
    Synonyms: CDH2, CDHN, NCAD, Neural cadherin, CDw325, Cadherin N, N-Cadherin
    纯化方法
    Subsequent Ultrafiltration and Size Exclusion Chromatography.
    组件
    incl. positive Control
    免疫原
    Peptide conjugated to hemocyanin. Epitope: Cytoplasmic domain
    克隆位点
    7-00E-009
    亚型
    IgG1
    Top Product
    Discover our top product CDH2 Primary Antibody
  • 应用备注
    Immunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Included Positive Control: Cell lysate from untreated SH-SY5Y cells (See Ptotocols for moredetails).
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    实验流程
    Positive Control: Cell lysate from untreated SH-SY5Y cellsFormat: Lyophilized cell lysate from serum starved SH-SY5Y cells. Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20. 000 cells. Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended forapplications with native proteins such as in immunoprecipitation.
    限制
    仅限研究用
  • 浓度
    0.5 mg/mL
    缓冲液
    PBS / 0.09 % Sodium Azide / PEG and Sucrose.
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
    储存方法
    Aliquote and freeze in liquid nitrogen. Antibody can be stored frozen at -80 °C up to 1 year. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
    有效期
    12 months
  • 抗原
    N-Cadherin (CDH2) (Cadherin 2 (CDH2))
    别名
    Cadherin-2 (CDH2 产品)
    别名
    CD325 antibody, CDHN antibody, CDw325 antibody, NCAD antibody, N-cadherin antibody, cadherin-2 antibody, cdhn antibody, ncad antibody, cdw325 antibody, CDH2 antibody, Cadherin-2 antibody, Ncad antibody, glo antibody, lyr antibody, pac antibody, wu:fb47h04 antibody, cadherin 2 antibody, cadherin 2 S homeolog antibody, cadherin 2, type 1, N-cadherin (neuronal) antibody, CDH2 antibody, cdh2.S antibody, cdh2 antibody, Cdh2 antibody
    背景
    Cadherins are a family of transmembrane glycoproteins that play a key role in calcium-dependent cell-cell adhesion. Several members of the cadherin family have been identified so far, including E- (epithelial), P- (placental), N- (neuronal), and M- (muscle) cadherin.Synonyms: CDH2, CDHN, CDw325, Cadherin N, N-Cadherin, NCAD, Neural cadherin
    基因ID
    1000
    UniProt
    P19022
    途径
    Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Synaptic Membrane
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