Topoisomerase I 抗体
Quick Overview for Topoisomerase I 抗体 (ABIN4914765)
抗原
See all Topoisomerase I (TOP1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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交叉反应
- 人, 大鼠
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纯化方法
- Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
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免疫原
- This TOP1 antibody is generated from a mouse immunized with a recombinant protein from the N-terminal region of human TOP1.
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亚型
- IgG1
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anti-Topoisomerase (DNA) I (TOP1) (AA 692-765) antibody
TOP1 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 1A1 unconjugated
anti-Topoisomerase (DNA) I (TOP1) antibodyTOP1 适用: 人 WB, IHC, IP, IF 宿主: 兔 Monoclonal unconjugated
anti-Topoisomerase (DNA) I (TOP1) antibodyTOP1 适用: 人 WB, ELISA, IHC, IP, FACS 宿主: 兔 Monoclonal 6D8 unconjugated Recombinant Antibody
anti-Topoisomerase (DNA) I (TOP1) antibodyTOP1 适用: 人, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) I (TOP1) (Internal Region) antibodyTOP1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) I (TOP1) (AA 1-100) antibodyTOP1 适用: 人 WB, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) I (TOP1) (N-Term) antibodyTOP1 适用: 人 WB, FACS 宿主: 小鼠 Monoclonal 1291CT875-142-166 unconjugated
anti-Topoisomerase (DNA) I (TOP1) (AA 358-501) antibodyTOP1 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Topoisomerase (DNA) I (TOP1) (AA 692-765) antibodyTOP1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Topoisomerase (DNA) I (TOP1) (AA 715-765) antibodyTOP1 适用: 人, 小鼠, Cow, 豚鼠, 马, 兔, 犬, Pig WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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WB 1:300-5000
FCM 1:20-100 -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 μg/μL
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缓冲液
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
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有效期
- 12 months
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- Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))
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别名
- TOP1
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背景
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Synonyms: TOPI, DNA topoisomerase 1, DNA topoisomerase I, TOP1
Background: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter.
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基因ID
- 7150
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UniProt
- P11387
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途径
- Caspase Cascade in Apoptosis, Stem Cell Maintenance
抗原
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