FGF6 抗体
Quick Overview for FGF6 抗体 (ABIN490848)
抗原
See all FGF6 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Recognizes human, recombinant Fibroblast Growth Factor 6 (FGF6). ~50 % cross-reactivity with rhFGF4, less than 5 % cross-reactivity with rhFGF9 and less than 2 % cross-reactivity with FGF-acidic, rhFGF5 and rhFGF7. In direct ELISA, this antibody shows no cross-reactivity with other cytokines tested.
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纯化方法
- Immunoaffinity purified
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免疫原
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Human, recombinant Fibroblast Growth Factor 6 (FGF6) from E. coli.
Type of Immunogen: Recombinant protein -
亚型
- IgG
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应用备注
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Approved: ELISA (0.5 - 1 μg/mL), Func, Neut, WB (0.1 - 0.2 μg/mL)
Usage: Suitable for use in Neutralization, Western Blot and ELISA. Neutralization: ND50 is ~0.025-0.075 μg/mL in the presence of 1 ng/mL of rhFGF6 and 1 μg/mL of heparin, using the FGF6 responsive NR6R-3T3 fibroblasts as target cells. The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when that cytokine is present at a concentration just high enough to elicit a maximum response. Western Blot: 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human FGF6. The detection limit for rhFGF6 is approximately 2 ng/lane under non-reducing and reducing conditions. ELISA: 0.5-1 μg/mL with the appropriate secondary reagents to detect human FGF6. The detection limit for rhFGF6 is ~0.16 ng/well. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, pH 7.2, 40 % glycerol.
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- FGF6 (Fibroblast Growth Factor 6 (FGF6))
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别名
- FGF6
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背景
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Name/Gene ID: FGF6
Family: HBGF
Synonyms: FGF6, FGF-6, Fibroblast growth factor 6, HSTF-2, HBGF-6, HST-2, HST2, HSTF2 -
基因ID
- 2251
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UniProt
- P10767
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途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
抗原
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