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FGF6 抗体

This anti-FGF6 antibody is a 山羊 多克隆 antibody detecting FGF6 in WB, ELISA, Neut 和 Func. Suitable for 人.
产品编号 ABIN490848
发货至: 中国

Quick Overview for FGF6 抗体 (ABIN490848)

抗原

See all FGF6 抗体
FGF6 (Fibroblast Growth Factor 6 (FGF6))

适用

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宿主

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山羊

克隆类型

  • 45
  • 6
多克隆

标记

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This FGF6 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Neutralization (Neut), Functional Studies (Func)
  • 特异性

    Recognizes human, recombinant Fibroblast Growth Factor 6 (FGF6). ~50 % cross-reactivity with rhFGF4, less than 5 % cross-reactivity with rhFGF9 and less than 2 % cross-reactivity with FGF-acidic, rhFGF5 and rhFGF7. In direct ELISA, this antibody shows no cross-reactivity with other cytokines tested.

    纯化方法

    Immunoaffinity purified

    免疫原

    Human, recombinant Fibroblast Growth Factor 6 (FGF6) from E. coli.

    Type of Immunogen: Recombinant protein

    亚型

    IgG
  • 应用备注

    Approved: ELISA (0.5 - 1 μg/mL), Func, Neut, WB (0.1 - 0.2 μg/mL)

    Usage: Suitable for use in Neutralization, Western Blot and ELISA. Neutralization: ND50 is ~0.025-0.075 μg/mL in the presence of 1 ng/mL of rhFGF6 and 1 μg/mL of heparin, using the FGF6 responsive NR6R-3T3 fibroblasts as target cells. The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when that cytokine is present at a concentration just high enough to elicit a maximum response. Western Blot: 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human FGF6. The detection limit for rhFGF6 is approximately 2 ng/lane under non-reducing and reducing conditions. ELISA: 0.5-1 μg/mL with the appropriate secondary reagents to detect human FGF6. The detection limit for rhFGF6 is ~0.16 ng/well.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS,  pH 7.2, 40 % glycerol.

    注意事项

    Avoid repeat freeze-thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • 抗原

    FGF6 (Fibroblast Growth Factor 6 (FGF6))

    别名

    FGF6

    背景

    Name/Gene ID: FGF6
    Family: HBGF

    Synonyms: FGF6, FGF-6, Fibroblast growth factor 6, HSTF-2, HBGF-6, HST-2, HST2, HSTF2

    基因ID

    2251

    UniProt

    P10767

    途径

    RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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