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Nucleolin 抗体 (pThr84)

This 兔 多克隆 anti-Nucleolin antibody specifically detects Nucleolin in WB, IF 和 IP. The antibody is reactive with 人 和 小鼠 samples.
产品编号 ABIN4889383
发货至: 中国
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Quick Overview for Nucleolin 抗体 (pThr84) (ABIN4889383)

抗原

See all Nucleolin (NCL) 抗体
Nucleolin (NCL)

适用

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人, 小鼠

宿主

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克隆类型

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多克隆

标记

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This Nucleolin antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
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    pThr84

    特异性

    Reacts with Nucleolin with phoshothreonine 84 (pT84) from human and mouse

    交叉反应 (详细)

    Cross reacts with phoshothreonine 106 (pT106). Do not cross react with phoshothreonine 76 & 92 (pT76 and pT92). The detection of P-NCL can be done during mitosis or in G2 phase, no signal in G1S phase.

    纯化方法

    Purified (protein A)

    免疫原

    Synthetic peptide of human Nucleolin with a phoshothreonine at position 84
  • 应用备注

    Working dilution: Optimal dilutions should be determined by the end user.
    The following are guidelines only:
    - WB : 1/200 to 1/2000 - IF: 1/200 to 1/1000

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Must be reconstituted in distilled water.

    浓度

    1 mg/mL

    缓冲液

    Tris 0,1M, glycine 0,1M

    储存条件

    4 °C/-20 °C

    储存方法

    Short term storage at+4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt.

    有效期

    6 months
  • 抗原

    Nucleolin (NCL)

    别名

    Nucleolin

    背景

    Ucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.

    基因ID

    4691

    UniProt

    P19338

    途径

    Ribonucleoprotein Complex Subunit Organization
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