Phosphotyrosine 抗体
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北京 101111
Quick Overview for Phosphotyrosine 抗体 (ABIN488674)
抗原
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Antibody reacts with phosphotyrosine and detects the presence ofphosphotyrosine in proteins of both unstimulated and stimulated cell lysated.
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交叉反应 （详细）
- Does not cross react with phosphoserine or phosphothreonine
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预测反应
- antibody is not species specific
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纯化方法
- affinity purified
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免疫原
- Phosphotyrosine, alanine and glyceine in a 1:1:1 ratio polymerized in the presence of keyhole limpet hemocyanin KLH with 1-ethyl-3-(3-™-dimentrylaminopropyl) carbodiimide
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亚型
- IgG1
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anti-Phosphotyrosine antibody
适用: Various Species WB, FACS, ICC 宿主: 小鼠 Monoclonal P-Tyr-01 unconjugated
anti-Phosphotyrosine antibody适用: Various Species WB, IHC (p) 宿主: 小鼠 Monoclonal 3F6 unconjugated
anti-Phosphotyrosine antibody (PerCP)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 PerCP
anti-Phosphotyrosine antibody (HRP)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 HRP
anti-Phosphotyrosine antibody (Atto 594)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 Atto 594
anti-Phosphotyrosine antibody (Biotin)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 Biotin
anti-Phosphotyrosine antibody (FITC)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 FITC
anti-Phosphotyrosine antibody (APC)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 APC
anti-Phosphotyrosine antibody (PE)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 PE
anti-Phosphotyrosine antibody (Atto 390)WB, IP, IF, IHC, ICC 宿主: 小鼠 Monoclonal G104 Atto 390
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应用备注
- 1 : 1000 with standard ECL (WB)
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说明
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1 μg/ml of this antibody is sufficient for detection of phosphorylated tyrosine residues in 10 μg of rat tissue lysate by colorimetric immunoblot analysis.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- protein G purified IgG1 in PBS, pH 7.4 with 0.09 % sodium azide and 50 % glycerol
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Make aliquots to avoid repeated freeze-thaw cycles.Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of thetubes.
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储存条件
- -20 °C
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储存方法
- store at -20°C for one year, make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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有效期
- 12 months
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: "Regulation of fibroblast motility by the protein tyrosine phosphatase PTP-PEST." in: The Journal of biological chemistry, Vol. 274, Issue 6, pp. 3811-8, (1999) (PubMed).
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- Phosphotyrosine
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物质类
- Amino Acid
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背景
- Tyrosine phosphorylation is considered to be one of the key steps in signal transduction and regulation of enzymatic activity. Phosphotyrosine antibodies are helpful in facilitating the identification of tyrosine kinase substrates.
抗原
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