NFIA 抗体 (Middle Region)
Quick Overview for NFIA 抗体 (Middle Region) (ABIN4886680)
抗原
See all NFIA 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 180-224, Middle Region
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原理
- Anti-NFIA Antibody Picoband®
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序列
- AYFVHAADSS QSESPSQPSD ADIKDQPENG HLGFQDSFVT SG
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-NFIA Antibody Picoband® (ABIN4886680). Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence in the middle region of human NFIA, different from the related mouse sequence by one amino acid, and identical to the related rat sequence.
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亚型
- IgG
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anti-Nuclear Factor I/A (NFIA) (AA 1-498) antibody
NFIA 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1E11 unconjugated
anti-Nuclear Factor I/A (NFIA) (AA 180-330) antibodyNFIA 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor I/A (NFIA) (AA 180-224) antibodyNFIA 适用: 人, 小鼠, 大鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor I/A (NFIA) (AA 1-498) antibodyNFIA 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor I/A (NFIA) (AA 348-397) antibodyNFIA 适用: 人, 小鼠, 大鼠, Cow, 马, 兔, 豚鼠, Pig, 小鸡, 猴, 非洲爪蟾 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor I/A (NFIA) (AA 384-418), (C-Term) antibodyNFIA 适用: 人, 大鼠 WB 宿主: 兔 Polyclonal RB51284 unconjugated
anti-Nuclear Factor I/A (NFIA) (C-Term) antibodyNFIA 适用: 人, 小鼠, 大鼠, Cow, 马, 兔, 猴, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor I/A (NFIA) (AA 1-143) antibodyNFIA 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor I/A (NFIA) (Middle Region) antibodyNFIA 适用: 人, 小鼠, 大鼠, Cow, 马, 兔, 犬, 豚鼠, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor I/A (NFIA) (AA 180-330) antibodyNFIA 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Human, Mouse
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human
Immunocytochemistry, 0.5-1 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human1. Leahy P, Crawford DR, Grossman G, et al. (1999). "CREB binding protein coordinates the function of multiple transcription factors including nuclear factor I to regulate phosphoenolpyruvate carboxykinase (GTP) gene transcription.". J. Biol. Chem. 274 (13): 8813-22. 2. Qian F, Kruse U, Lichter P, Sippel AE (Dec 1995). "Chromosomal localization of the four genes (NFIA, B, C, and X) for the human transcription factor nuclear factor I by FISH". Genomics 28 (1): 66-73. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- NFIA (Nuclear Factor I/A (NFIA))
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别名
- NFIA
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背景
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Synonyms: Nuclear factor 1 A-type,NF1-A,Nuclear factor 1/A,CCAAT-box-binding transcription factor,CTF,Nuclear factor I/A,NF-I/A,NFI-A,TGGCA-binding protein,NFIA,KIAA1439,
Tissue Specificity: Ubiquitous. .
Background: Nuclear factor 1 A-type is a protein that in humans is encoded by the NFIA gene. Nuclear factor I (NFI) proteins constitute a family of dimericDNA-binding proteins with similar, and possibly identical, DNA-binding specificity. They function as cellular transcription factors and as replication factors for adenovirus DNA replication. Diversity in this protein family is generated by multiple genes, differential splicing, and heterodimerization.
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分子量
- 69 kDa
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基因ID
- 4774
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UniProt
- Q12857
抗原
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