GPI 抗体 (N-Term)
Quick Overview for GPI 抗体 (N-Term) (ABIN4886608)
抗原
See all GPI 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 5-39, N-Term
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原理
- Anti-Glucose 6 phosphate isomerase/GPI Antibody Picoband®
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序列
- TRDPQFQKLQ QWYREHRSEL NLRRLFDANK DRFNH
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交叉反应 (详细)
- No cross-reactivity with other proteins.
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产品特性
- Anti-Glucose 6 phosphate isomerase/GPI Antibody Picoband® (ABIN4886608). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of human GPI, different from the related mouse and rat sequences by sixteen amino acids.
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亚型
- IgG
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human
1. Haga A, Niinaka Y, Raz A (2000). "Phosphohexose isomerase/autocrine motility factor/neuroleukin/maturation factor is a multifunctional phosphoprotein.". Biochim. Biophys. Acta 1480(1-2): 235-44. 2. Kugler W, Lakomek M (March 2000). "Glucose-6-phosphate isomerase deficiency". Baillieres Best Pract. Res. Clin. Haematol. 13 (1): 89-101. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- GPI (Glucose-6-Phosphate Isomerase (GPI))
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别名
- GPI
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背景
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Synonyms: Glucose-6-phosphate isomerase,GPI,5.3.1.9,Autocrine motility factor,AMF,Neuroleukin,NLK,Phosphoglucose isomerase,PGI,Phosphohexose isomerase,PHI,Sperm antigen 36,SA-36,GPI,
Tissue Specificity: Expressed in heart, spleen, testis and, to a lower extent, in thymus and peripheral blood leukocytes. Weakly expressed in lung, placenta, colon and small intestine.
Background: Glucose-6-phosphate isomerase (GPI), alternatively known as phosphoglucose isomerase (PGI) or phosphohexose isomerase (PHI), is an enzyme that in humans is encoded by the GPI gene on chromosome 19. This gene encodes a member of the glucose phosphate isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In the cytoplasm, the gene product functions as a glycolytic enzyme (glucose-6-phosphate isomerase) that interconverts glucose-6-phophsate and fructose-6-phosphate. Extracellularly, the encoded protein (also referred to as neuroleukin) functions as a neurotrophic factor that promotes survival of skeletal motor neurons and sensory neurons, and as a lymphokine that induces immunoglobulin secretion. The encoded protein is also referred to as autocrine motility factor based on an additional function as a tumor-secreted cytokine and angiogenic factor. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. Alternative splicing results in multiple transcript variants.
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分子量
- 63 kDa
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基因ID
- 2821
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UniProt
- P06744
抗原
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