CD33 抗体 (AA 18-259)
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- 抗原 See all CD33 抗体
- CD33
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抗原表位
- AA 18-259
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CD33 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
- 原理
- Anti-CD33 Antibody Picoband®
- 交叉反应 (详细)
- No cross-reactivity with other proteins
- 产品特性
- Anti-CD33 Antibody Picoband® (ABIN4886523). Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- 纯化方法
- Immunogen affinity purified.
- 免疫原
- E. coli-derived human CD33 recombinant protein (Position: D18-H259). Human CD33 shares 61.6% amino acid (aa) sequence identity with mouse CD33.
- 亚型
- IgG
- Top Product
- Discover our top product CD33 Primary Antibody
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- 应用备注
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Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL
Immunocytochemistry, 0.5-1 μg/mL
Western blot, 0.1-0.5 μg/mL
Flow Cytometry, 1-3 μg/1x106cells
1. Adriaansen, H. J., Geurts Van Kessel, A. H. M., Wijdenes-De Bresser, J. H. F. M., Van Drunen-Schoenmaker, E., Van Dongen, J. J. M.Expression of the myeloid differentiation antigen CD33 depends on the presence of human chromosome 19 in human-mouse hybrids. Ann. Hum. Genet. 54: 115-119, 1990. 2. Brinkman-Van der Linden, E. C. M., Angata, T., Reynolds, S. A., Powell, L. D., Hedrick, S. M., Varki, A. CD33/Siglec-3 binding specificity, expression pattern, and consequences of gene deletion in mice. Molec. Cell. Biol. 23: 4199-4206, 2003. 3. Trask, B., Fertitta, A., Christensen, M., Youngblom, J., Bergmann, A., Copeland, A., de Jong, P., Mohrenweiser, H., Olsen, A., Carrano, A., Tynan, K. Fluorescence in situ hybridization mapping of human chromosome 19: cytogenetic band location of 540 cosmids and 70 genes or DNA markers. Genomics 15: 133-145, 1993. - 说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- 抗原
- CD33
- 别名
- CD33 (CD33 产品)
- 背景
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Synonyms: Myeloid cell surface antigen CD33,Sialic acid-binding Ig-like lectin 3,Siglec-3,gp67,CD33,CD33,SIGLEC3,
Tissue Specificity: Monocytic/myeloid lineage cells.
Background: CD33, also known as Siglec-3 (sialic acid binding Ig-like lectin 3, SIGLEC3, SIGLEC-3, gp67, p67), is a transmembrane receptor expressed on cells of myeloid lineage. It is usually considered myeloid-specific, but it can also be found on some lymphoid cells. CD33 binds sialic acids, therefore is a member of the SIGLEC family of lectins. By fluorescence in situ hybridization, CD33 is mapped to 19q13.3-q13.4.
- 分子量
- 45 kDa
- 基因ID
- 945
- UniProt
- P20138
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