Calpastatin 抗体 (Middle Region)
Quick Overview for Calpastatin 抗体 (Middle Region) (ABIN4886503)
抗原
See all Calpastatin (CAST) 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 275-310, Middle Region
-
原理
- Anti-Calpastatin/CAST Antibody Picoband®
-
序列
- QEKKRKVEKD TMSDQALEAL SASLGTRQAE PELDLR
-
交叉反应 （详细）
- No cross-reactivity with other proteins
-
产品特性
- Anti-Calpastatin/CAST Antibody Picoband® (ABIN4886503). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
-
纯化方法
- Immunogen affinity purified.
-
免疫原
- A synthetic peptide corresponding to a sequence in the middle region of human Calpastatin, different from the related mouse sequence by fifteen amino acids, and from the related rat sequence by eleven amino acids.
-
亚型
- IgG
-
-
anti-Calpastatin (CAST) antibody
CAST 适用: 人 ELISA, IHC, Coat, StM 宿主: 小鼠 Monoclonal CAST-1550 unconjugated
anti-Calpastatin (CAST) (AA 1-667) antibodyCAST 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Calpastatin (CAST) (AA 582-669) antibodyCAST 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2C5 unconjugated
anti-Calpastatin (CAST) (AA 275-310) antibodyCAST 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal unconjugated
anti-Calpastatin (CAST) (AA 1-350) antibodyCAST 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Calpastatin (CAST) (AA 431-530) antibodyCAST 适用: 人, 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Calpastatin (CAST) (AA 275-700) antibodyCAST 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Calpastatin (CAST) (AA 1-686) antibodyCAST 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Calpastatin (CAST) (AA 1-667) antibodyCAST 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Calpastatin (CAST) antibody (FITC)CAST 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal FITC
-
-
应用备注
-
Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human1. Averna M, De Tullio R, Capini P, Salamino F, Pontremoli S, Melloni E (Dec 2003). "Changes in calpastatin localization and expression during calpain activation: a new mechanism for the regulation of intracellular Ca(2+)-dependent proteolysis". Cell Mol Life Sci. 60 (12): 2669-78. 2. Ma H, Yang HQ, Takano E, Lee WJ, Hatanaka M, Maki M (Sep 1993). "Requirement of different subdomains of calpastatin for calpain inhibition and for binding to calmodulin-like domains". J Biochem. 113 (5): 591-9. 3. Raynaud P, Jayat-Vignoles C, Laforet MP, Leveziel H, Amarger V (Apr 2005). "Four promoters expression of the calpastatin gene". Arch Biochem Biophys. 437 (1): 69-77. -
说明
-
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
-
浓度
- 500 μg/mL
-
缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Avoid repeated freezing and thawing.
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
-
-
- Calpastatin (CAST)
-
别名
- CAST
-
背景
-
Synonyms: Calpastatin,Calpain inhibitor,Sperm BS-17 component,CAST,
Tissue Specificity: Expressed in the islets of Langerhans (at protein level).
Background: Calpastatin is a protein that in humans is encoded by the CAST gene. It is mapped to 5q15. The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described.
-
分子量
- 120 kDa
-
基因ID
- 831
-
UniProt
- P20810
抗原
-
