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Calpastatin 抗体 (Middle Region)

Name: Calpastatin 抗体 (Middle Region)
SKU: ABIN4886503
Availability: OutOfStock

CAST 适用: 人 WB, IHC, IF, FACS, ICC 宿主: 兔 Polyclonal unconjugated

Western blot analysis of Calpastatin using anti-Calpastatin antibody . (Calpastatin 抗体 (Middle Region))

IHC analysis of Calpastatin using anti-Calpastatin antibody . (Calpastatin 抗体 (Middle Region))

IHC

3 images

产品编号 ABIN4886503

发货至: 中国

Datasheet as PDF

抗原 See all Calpastatin (CAST) 抗体

Calpastatin (CAST)
抗原表位
14

7

3

2

1

1

1

1

1

1

1

1

1
AA 275-310, Middle Region
适用
48

18

3

2
人
宿主
38

12
兔
Compatible Secondaries
克隆类型
41

9
多克隆
标记
25

4

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This Calpastatin antibody is un-conjugated
应用范围
37

15

12

12

7

6

6

4

3

2

2

2

1

1
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

原理

Anti-Calpastatin/CAST Antibody Picoband®

序列

QEKKRKVEKD TMSDQALEAL SASLGTRQAE PELDLR

交叉反应（详细）

No cross-reactivity with other proteins

产品特性

Anti-Calpastatin/CAST Antibody Picoband® (ABIN4886503). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

纯化方法

Immunogen affinity purified.

免疫原

A synthetic peptide corresponding to a sequence in the middle region of human Calpastatin, different from the related mouse sequence by fifteen amino acids, and from the related rat sequence by eleven amino acids.

亚型

IgG

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CAST 适用: 人 ELISA, IHC, Coat, StM 宿主: 小鼠 Monoclonal CAST-1550 unconjugated

anti-Calpastatin (CAST) (AA 1-667) antibody
CAST 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Calpastatin (CAST) (AA 582-669) antibody
CAST 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2C5 unconjugated

anti-Calpastatin (CAST) (AA 275-310) antibody
CAST 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal unconjugated

anti-Calpastatin (CAST) (AA 1-350) antibody
CAST 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Calpastatin (CAST) (AA 431-530) antibody
CAST 适用: 人, 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Calpastatin (CAST) (AA 1-686) antibody
CAST 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated

anti-Calpastatin (CAST) (AA 1-667) antibody
CAST 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated

anti-Calpastatin (CAST) antibody (FITC)
CAST 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal FITC

anti-Calpastatin (CAST) (AA 550-650) antibody
CAST 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

应用备注

Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. Averna M, De Tullio R, Capini P, Salamino F, Pontremoli S, Melloni E (Dec 2003). "Changes in calpastatin localization and expression during calpain activation: a new mechanism for the regulation of intracellular Ca(2+)-dependent proteolysis". Cell Mol Life Sci. 60 (12): 2669-78. 2. Ma H, Yang HQ, Takano E, Lee WJ, Hatanaka M, Maki M (Sep 1993). "Requirement of different subdomains of calpastatin for calpain inhibition and for binding to calmodulin-like domains". J Biochem. 113 (5): 591-9. 3. Raynaud P, Jayat-Vignoles C, Laforet MP, Leveziel H, Amarger V (Apr 2005). "Four promoters expression of the calpastatin gene". Arch Biochem Biophys. 437 (1): 69-77.

说明

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

限制

仅限研究用
状态

Lyophilized

溶解方式

Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

浓度

500 μg/mL

缓冲液

Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C,-20 °C

储存方法

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
抗原

Calpastatin (CAST)

别名

CAST (CAST 产品)

背景

Synonyms: Calpastatin,Calpain inhibitor,Sperm BS-17 component,CAST,
Tissue Specificity: Expressed in the islets of Langerhans (at protein level).
Background: Calpastatin is a protein that in humans is encoded by the CAST gene. It is mapped to 5q15. The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described.

分子量

120 kDa

基因ID

831

UniProt

P20810