ARFGAP1 抗体 (AA 1-183)
Quick Overview for ARFGAP1 抗体 (AA 1-183) (ABIN4886474)
抗原
See all ARFGAP1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-183
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原理
- Anti-ARFGAP1 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-ARFGAP1 Antibody Picoband® (ABIN4886474). Tested in Flow Cytometry, ICC, IHC, IF, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E. coli-derived human ARFGAP1 recombinant protein (Position: M1-Y183). Human ARFGAP1 shares 89.1% and 88.5% amino acid (aa) sequence identity with mouse and rat ARFGAP1, respectively.
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亚型
- IgG
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anti-ADP-Ribosylation Factor GTPase Activating Protein 1 (ARFGAP1) antibody
ARFGAP1 适用: 人, 猴 WB, IHC, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal 1F6 unconjugated
anti-ADP-Ribosylation Factor GTPase Activating Protein 1 (ARFGAP1) antibodyARFGAP1 适用: 人, 猴 WB, IF, FACS 宿主: 小鼠 Monoclonal 1H6 unconjugated
anti-ADP-Ribosylation Factor GTPase Activating Protein 1 (ARFGAP1) (AA 270-414) antibodyARFGAP1 适用: 人, 小鼠 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 1C4E2 unconjugated
anti-ADP-Ribosylation Factor GTPase Activating Protein 1 (ARFGAP1) antibodyARFGAP1 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-ADP-Ribosylation Factor GTPase Activating Protein 1 (ARFGAP1) (AA 377-406), (C-Term) antibodyARFGAP1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB4855 unconjugated
anti-ADP-Ribosylation Factor GTPase Activating Protein 1 (ARFGAP1) (AA 1-220) antibodyARFGAP1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-ADP-Ribosylation Factor GTPase Activating Protein 1 (ARFGAP1) (Internal Region) antibodyARFGAP1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-ADP-Ribosylation Factor GTPase Activating Protein 1 (ARFGAP1) antibodyARFGAP1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-ADP-Ribosylation Factor GTPase Activating Protein 1 (ARFGAP1) antibodyARFGAP1 适用: 人, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-ADP-Ribosylation Factor GTPase Activating Protein 1 (ARFGAP1) (AA 287-336) antibodyARFGAP1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
1. "Entrez Gene: ARFGAP1 ADP-ribosylation factor GTPase activating protein 1". 2. Antonny, Bruno (June 2011). "Mechanisms of membrane curvature sensing". Annu Rev Biochem. 80: 101-123. 3. Huber I, Rotman M, Pick E, Makler V, Rothem L, Cukierman E, Cassel D (February 2001). "Expression, purification, and properties of ADP-ribosylation factor (ARF) GTPase activating protein-1". Methods Enzymol. 329: 307-16. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- ARFGAP1 (ADP-Ribosylation Factor GTPase Activating Protein 1 (ARFGAP1))
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别名
- ARFGAP1
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背景
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Synonyms: ADP-ribosylation factor GTPase-activating protein 1,ARF GAP 1,ADP-ribosylation factor 1 GTPase-activating protein,ARF1 GAP,ARF1-ed GTPase-activating protein,ARFGAP1,ARF1GAP,
Tissue Specificity: Ubiquitous. .
Background: ADP-ribosylation factor GTPase-activating protein 1 is an enzyme that in humans is encoded by the ARFGAP1 gene. And this gene is mapped to 20q13.33. The protein encoded by this gene is a GTPase-activating protein, which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Alternative splicing results in multiple transcript variants.
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分子量
- 45 kDa
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基因ID
- 55738
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UniProt
- Q8N6T3
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途径
- p53 Pathway, ER-Nucleus Signaling, Unfolded Protein Response
抗原
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