APRT 抗体 (N-Term)
Quick Overview for APRT 抗体 (N-Term) (ABIN4886471)
抗原
See all APRT 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 5-49, N-Term
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原理
- Anti-APRT Antibody
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序列
- ELQLVEQRIR SFPDFPTPGV VFRDISPVLK DPASFRAAIG LLARH
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交叉反应 (详细)
- No cross-reactivity with other proteins.
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产品特性
- Anti-APRT Antibody (ABIN4886471). Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of human APRT, different from the related mouse sequence by ten amino acids, and from the related rat sequence by nine amino acids.
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亚型
- IgG
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- APRT (Adenine Phosphoribosyltransferase (APRT))
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别名
- APRT
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背景
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Synonyms: Adenine phosphoribosyltransferase,APRT,2.4.2.7,APRT,
Tissue Specificity: Expressed in brain with higher levels in fetal brain. Found in cerebellum and occipital pole.
Background: Adenine phosphoribosyltransferase (APRTase) is an enzyme encoded by the APRT gene, found in humans on chromosome 16. It belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene.
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分子量
- 20 kDa
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基因ID
- 353
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UniProt
- P07741
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途径
- Ribonucleoside Biosynthetic Process
抗原
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