APRT 抗体 (N-Term)
Quick Overview for APRT 抗体 (N-Term) (ABIN4886471)
抗原
See all APRT 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 5-49, N-Term
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原理
- Anti-APRT Antibody Picoband®
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序列
- ELQLVEQRIR SFPDFPTPGV VFRDISPVLK DPASFRAAIG LLARH
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-APRT Antibody Picoband® (ABIN4886471). Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of human APRT, different from the related mouse sequence by ten amino acids, and from the related rat sequence by nine amino acids.
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亚型
- IgG
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anti-Adenine Phosphoribosyltransferase (APRT) (AA 12-40), (N-Term) antibody
APRT 适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB20509 unconjugated
anti-Adenine Phosphoribosyltransferase (APRT) (AA 1-180) antibodyAPRT 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Adenine Phosphoribosyltransferase (APRT) (AA 1-180) antibodyAPRT 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Adenine Phosphoribosyltransferase (APRT) (AA 143-170), (C-Term) antibodyAPRT 适用: 人, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB20510 unconjugated
anti-Adenine Phosphoribosyltransferase (APRT) (AA 149-179) antibodyAPRT 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Adenine Phosphoribosyltransferase (APRT) (Internal Region) antibodyAPRT 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Adenine Phosphoribosyltransferase (APRT) (AA 1-180) antibodyAPRT 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Adenine Phosphoribosyltransferase (APRT) antibodyAPRT 适用: 人 WB, IHC, FACS, IF/ICC 宿主: 兔 Polyclonal unconjugated
anti-Adenine Phosphoribosyltransferase (APRT) (AA 1-180) antibodyAPRT 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Adenine Phosphoribosyltransferase (APRT) (N-Term) antibodyAPRT 适用: 人, 大鼠, 犬 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human1. Silva CH, Silva M, Iulek J, Thiemann OH (Jun 2008). "Structural complexes of human adenine phosphoribosyltransferase reveal novel features of the APRT catalytic mechanism". Journal of Biomolecular Structure & Dynamics. 25 (6): 589-97. 2. Sarver AE, Wang CC (Oct 2002). "The adenine phosphoribosyltransferase from Giardia lamblia has a unique reaction mechanism and unusual substrate binding properties". The Journal of Biological Chemistry. 277(42): 39973-80. 3. Valaperta R, Rizzo V, Lombardi F, Verdelli C, Piccoli M, Ghiroldi A, Creo P, Colombo A, Valisi M, Margiotta E, Panella R, Costa E (1 July 2014). "Adenine phosphoribosyltransferase (APRT) deficiency: identification of a novel nonsense mutation". BMC Nephrology. 15: 102. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- APRT (Adenine Phosphoribosyltransferase (APRT))
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别名
- APRT
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背景
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Synonyms: Adenine phosphoribosyltransferase,APRT,2.4.2.7,APRT,
Tissue Specificity: Expressed in brain with higher levels in fetal brain. Found in cerebellum and occipital pole.
Background: Adenine phosphoribosyltransferase (APRTase) is an enzyme encoded by the APRT gene, found in humans on chromosome 16. It belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene.
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分子量
- 20 kDa
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基因ID
- 353
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UniProt
- P07741
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途径
- Ribonucleoside Biosynthetic Process
抗原
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