Adenylate Kinase 2 抗体 (AA 161-239)
Quick Overview for Adenylate Kinase 2 抗体 (AA 161-239) (ABIN4886446)
抗原
See all Adenylate Kinase 2 (AK2) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 161-239
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原理
- Anti-AK2 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-AK2 Antibody Picoband® (ABIN4886446). Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E. coli-derived human AK2 recombinant protein (Position: E161-I239). Human AK2 shares 93.7% and 93% amino acid (aa) sequence identity with mouse and rat AK2, respectively.
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亚型
- IgG
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anti-Adenylate Kinase 2 (AK2) (AA 147-183) antibody
AK2 适用: 人, 小鼠, 斑马鱼 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB53214 unconjugated
anti-Adenylate Kinase 2 (AK2) (AA 1-232) antibodyAK2 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Adenylate Kinase 2 (AK2) (AA 64-232) antibodyAK2 适用: 大鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Adenylate Kinase 2 (AK2) antibodyAK2 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Adenylate Kinase 2 (AK2) (N-Term) antibodyAK2 适用: 斑马鱼 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB53220 unconjugated
anti-Adenylate Kinase 2 (AK2) (AA 1-239) antibodyAK2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Adenylate Kinase 2 (AK2) (AA 1-30), (N-Term) antibodyAK2 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB3893 unconjugated
anti-Adenylate Kinase 2 (AK2) (AA 187-217), (C-Term) antibodyAK2 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB3896 unconjugated
anti-Adenylate Kinase 2 (AK2) (AA 2-36), (N-Term) antibodyAK2 适用: 斑马鱼 WB 宿主: 兔 Polyclonal RB46461 unconjugated
anti-Adenylate Kinase 2 (AK2) (AA 1-239) antibodyAK2 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
1. Bruns GA, Regina VM (1977). "Adenylate kinase 2, a mitochondrial enzyme". Biochem. Genet. 15 (5-6): 477-86. 2. Köhler C, Gahm A, Noma T, et al. (1999). "Release of adenylate kinase 2 from the mitochondrial intermembrane space during apoptosis". FEBS Lett. 447 (1): 10-2. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- Adenylate Kinase 2 (AK2)
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别名
- AK2
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背景
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Synonyms: Adenylate kinase 2, mitochondrial ,AK 2 ,2.7.4.3 ,ATP-AMP transphosphorylase 2 ,ATP:AMP phosphotransferase ,Adenylate monophosphate kinase ,Adenylate kinase 2, mitochondrial, N-terminally processed ,AK2 ,ADK2,
Tissue Specificity: Present in most tissues. Present at high level in heart, liver and kidney, and at low level in brain, skeletal muscle and skin. Present in thrombocytes but not in erythrocytes, which lack mitochondria. Present in all nucleated cell populations from blood, while AK1 is mostly absent. In spleen and lymph nodes, mononuclear cells lack AK1, whereas AK2 is readily detectable. These results indicate that leukocytes may be susceptible to defects caused by the lack of AK2, as they do not express AK1 in sufficient amounts to compensate for the AK2 functional deficits (at protein level). .
Background: Adenylate kinase 2 is an enzyme is encoded in humans by the AK2 gene. The AK2 protein is found in the intermembrane space of the mitochondrion. Adenylate kinases are involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. Three isozymes of adenylate kinase, namely 1, 2, and 3, have been identified in vertebrates, this gene encodes isozyme 2. Expression of these isozymes is tissue-specific and developmentally regulated. Isozyme 2 is localized in the mitochondrial intermembrane space and may play a role in apoptosis. Mutations in this gene are the cause of reticular dysgenesis. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 1 and 2.
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分子量
- 26 kDa
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基因ID
- 204
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UniProt
- P54819
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途径
- Nucleotide Phosphorylation, Ribonucleoside Biosynthetic Process
抗原
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