ACAT1 抗体 (Internal Region)
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Quick Overview for ACAT1 抗体 (Internal Region) (ABIN487980)
抗原
See all ACAT1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 253-266, Internal Region
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特异性
- This antibody recognizes ACAT1 (253-266).
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交叉反应 (详细)
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Species reactivity (expected):Canine, Bovine.
Species reactivity (tested):Human, Mouse, Rat. -
纯化方法
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
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免疫原
- Peptide with sequence C-DEEYKRVDFSKVPK, from the internal region of the protein sequence according to NP_000010.1. Genename: ACAT1
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应用备注
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Peptide ELISA: 1/2000. Western blot: Approx 45 kDa band observed in Human, Mouse and Rat Liver lysates(calculated MW of 45.5 kDa according to Human NP_000010.1 and 44.85 kDa to MouseNP_659033.1). Recommended concentration: 0.1 - 0.3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- ACAT1 (Acetyl-CoA Acetyltransferase 1 (ACAT1))
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别名
- ACAT1 / MAT
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背景
- ACAT1, acetoacetyl CoA thiolase, plays a major role in ketone body metabolism. It is a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in the gene encoding ACAT1 are associated with the alpha-methylacetoaceticaciduria disorder,an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.Synonyms: Acetoacetyl-CoA thiolase T2, Acetyl-CoA acetyltransferase mitochondrial
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基因ID
- 38
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NCBI登录号
- NP_000010
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UniProt
- P24752
抗原
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