LY9 抗体
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北京 101111
Quick Overview for LY9 抗体 (ABIN487455)
抗原
See all LY9 (CD229) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody reacts with Human Ly-9 antigen.
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交叉反应 (详细)
- Species reactivity (tested):Human.
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产品特性
- Synonyms: Ly-9, T-lymphocyte surface antigen Ly-9, Lymphocyte antigen 9
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免疫原
- Mouse pre-B-cell line 300. 19, stably transfected with the full length Human Ly-9 cDNA. Remarks: This antibody was concentrated from Hybridoma (clone HLy-9. 1. 25) supernatant. Hybridoma was established by fusion of mouse myeloma cell NS-1 with Balb/c mousesplenocyte.
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亚型
- IgG1
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应用备注
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Flow Cytometry: 10 μg/mLPositive Control: Peripheral blood T cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
实验流程
- Flow Cytometric Analysis for Floating CellsWe use Fisher tubes or equivalents as reaction tubes for all step described below. 1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS).
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS, 7.2 containing 50 % Glycerol without preservatives.
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储存液
- Without preservative
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储存条件
- 4 °C
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储存方法
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Store the antibody undiluted at 2-8 °C.
Shelf life: one year from despatch. -
有效期
- 12 months
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- LY9 (CD229) (Lymphocyte Antigen 9 (CD229))
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别名
- CD229 / LY9
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背景
- CD229 is a 100-120 kD cell surface glycoprotein that is the homologue of murine Ly-9. CD229 is a member of the immunoglobulin superfamily and may have a function in cell signalling. The antigen is expressed at low levels by mature T and B lymphocytes and at higher levels by single-positive thymocytes. Expression at variable levels is also seen in some cell lines, such as Daudi, U937 and HUT78.Synonyms: Ly-9, Lymphocyte antigen 9, T-lymphocyte surface antigen Ly-9
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基因ID
- 4063
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UniProt
- Q9HBG7
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途径
- BCR Signaling
抗原
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