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Nitrotyrosine 抗体

This anti-Nitrotyrosine antibody is a 小鼠 单克隆 antibody detecting Nitrotyrosine in WB, IP 和 ICC. Suitable for .
产品编号 ABIN479556
发货至: 中国

Quick Overview for Nitrotyrosine 抗体 (ABIN479556)

抗原

See all Nitrotyrosine 抗体
Nitrotyrosine

适用

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宿主

  • 24
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小鼠

克隆类型

  • 26
  • 24
单克隆

标记

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This Nitrotyrosine antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)
  • 特异性

    Recognizes nitrated proteins from all species.

    纯化方法

    Protein G purified

    免疫原

    Purified, nitrated Keyhole Limpet Hemocyanin (KLH).

    Type of Immunogen: Hapten

    亚型

    IgG2b kappa
  • 应用备注

    Approved: ICC (5 - 10 μg/mL), IP, WB (0.5 - 2 μg/mL)

    Usage: Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry. Western Blot: 0.5-2 μg/mL detects nitrotyrosine immunoblotting controls. A previous lot detected nitrated proteins in a lysate prepared from peroxynitrite-treated A431 cells. No reaction was noted with non-nitrated proteins. Immunoprecipitation: To be determined by the researcher. Immunocytochemistry: 5-10 μg/mL showed positive immunostaining for nitrated proteins in peroxynitrite-treated A431 cells fixed with 95 % ethanol/5 % acetic acid.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • 抗原

    Nitrotyrosine

    物质类

    Chemical
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