Nitrotyrosine 抗体
Quick Overview for Nitrotyrosine 抗体 (ABIN479556)
抗原
See all Nitrotyrosine 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Recognizes nitrated proteins from all species.
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纯化方法
- Protein G purified
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免疫原
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Purified, nitrated Keyhole Limpet Hemocyanin (KLH).
Type of Immunogen: Hapten -
亚型
- IgG2b kappa
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应用备注
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Approved: ICC (5 - 10 μg/mL), IP, WB (0.5 - 2 μg/mL)
Usage: Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry. Western Blot: 0.5-2 μg/mL detects nitrotyrosine immunoblotting controls. A previous lot detected nitrated proteins in a lysate prepared from peroxynitrite-treated A431 cells. No reaction was noted with non-nitrated proteins. Immunoprecipitation: To be determined by the researcher. Immunocytochemistry: 5-10 μg/mL showed positive immunostaining for nitrated proteins in peroxynitrite-treated A431 cells fixed with 95 % ethanol/5 % acetic acid. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- Nitrotyrosine
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物质类
- Chemical
抗原
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