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GSK3 alpha 抗体 (AA 203-219)

The 小鼠 单克隆 anti-GSK3 alpha antibody is suitable to detect GSK3 alpha in samples from Drosophila melanogaster. It has been validated for WB 和 ICC.
产品编号 ABIN470870
发货至: 中国
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Quick Overview for GSK3 alpha 抗体 (AA 203-219) (ABIN470870)

抗原

See all GSK3 alpha (GSK3a) 抗体
GSK3 alpha (GSK3a) (Glycogen Synthase Kinase 3 alpha (GSK3a))

适用

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Drosophila melanogaster

宿主

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小鼠

克隆类型

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单克隆

标记

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This GSK3 alpha antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunocytochemistry (ICC)
  • 抗原表位

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    AA 203-219

    特异性

    Recognizes Glycogen Synthase Kinase 3 (GSK3). Due to extremely high conservation among the known enzymes of many different species, should cross-react with nearly all species homologues. Species cross-reactivity: Mouse, rat, S. pombe, Dictyostelium and human.

    预测反应

    Percent identity by BLAST analysis: Drosophila, Mosquito (100%) Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Bat, Bovine, Horse, Rabbit, Opossum, Turkey, Chicken, Platypus, Xenopus, Stickleback, Pufferfish, Zebrafish (88%).

    纯化方法

    Protein G purified

    免疫原

    Synthetic peptide corresponding to aa203-219 (18aa, C-KQLLHGEPNVSYICSRY) derived from a region of the catalytic domain (between subdomain VII and VIII) of the Drosophila GSK-3/ shaggy enzyme (ovalbumin coupled). Percent identity by BLAST analysis: Drosophila, Mosquito (100%), Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Bat, Bovine, Horse, Rabbit, Opossum, Turkey, Chicken, Platypus, Xenopus, Stickleback, Pufferfish, Zebrafish (88%).

    Type of Immunogen: Synthetic peptide

    亚型

    IgG1
  • 应用备注

    Approved: ICC (10 μg/mL), WB (0.5 - 2 μg/mL)

    Usage: Suitable for use in Western Blot and Immunocytochemistry. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system. Immunocytochemistry: 10 μg/mL. Demonstrated positive immunostaining for GSK3 in L6 cells fixed with acetone. Positive control: J9000: Jurkat Cell.

    说明

    Target Species of Antibody: Drosophila

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeat freeze-thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • 抗原

    GSK3 alpha (GSK3a) (Glycogen Synthase Kinase 3 alpha (GSK3a))

    别名

    GSK3A / GSK3 Alpha

    背景

    Name/Gene ID: GSK3A
    Subfamily: GSK-3
    Family: Protein Kinase

    Synonyms: GSK3A, GSK-3 alpha, GSK3 alpha

    基因ID

    2931

    UniProt

    P49840

    途径

    PI3K-Akt Signaling, WNT signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, BCR Signaling, Warburg Effect
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