FZD3 抗体 (Cysteine-Rich Domain, Extracellular) (Biotin)
Quick Overview for FZD3 抗体 (Cysteine-Rich Domain, Extracellular) (Biotin) (ABIN470848)
抗原
See all FZD3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Cysteine-Rich Domain, Extracellular
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特异性
- Recognizes mouse Frizzled-3. Shows no cross-reactivity with rmFrizzled-1, -4, -7 and -8. Species cross-reactivity: Human.
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纯化方法
- Protein G purified
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免疫原
- NS0-derived rmFrizzled-3 extracellular CRD.
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亚型
- IgG2a
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anti-Frizzled Family Receptor 3 (FZD3) (AA 121-220) antibody
FZD3 适用: 人, 小鼠, 大鼠, 非洲爪蟾 WB, ELISA, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Frizzled Family Receptor 3 (FZD3) (AA 23-205) antibodyFZD3 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Frizzled Family Receptor 3 (FZD3) (N-Term) antibodyFZD3 适用: 人, 小鼠, 大鼠, Bat, Cow, 犬, Hamster, 马, 猴, Pig, 兔, 小鸡 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Frizzled Family Receptor 3 (FZD3) (AA 55-157) antibodyFZD3 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2H5 unconjugated
anti-Frizzled Family Receptor 3 (FZD3) (AA 55-157) antibodyFZD3 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1B8 unconjugated
anti-Frizzled Family Receptor 3 (FZD3) (Internal Region) antibodyFZD3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Frizzled Family Receptor 3 (FZD3) (Internal Region) antibodyFZD3 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Frizzled Family Receptor 3 (FZD3) (AA 55-157) antibodyFZD3 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Frizzled Family Receptor 3 (FZD3) (AA 581-596), (Intracellular) antibodyFZD3 适用: 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Frizzled Family Receptor 3 (FZD3) (AA 481-530) antibodyFZD3 适用: 人, 小鼠 WB, ELISA, IHC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: Flo, ICC, IHC, IHC-Fr, WB (1 - 2 μg/mL)
Usage: Suitable for use in Western Blot, Flow Cytometry and Immunocytochemistry. Detected Frizzled-3 by flow cytometry and immunohistochemistry on the differentiated embryoid bodies derived from the mouse embryonic cell line, D3. Immunocytochemistry and flow cytometry experiments on human HEK 293 cells demonstrate that this antibody cross-reacts with human Frizzled-3. Western Blot: Use at 1-2 μg/mL with the appropriate secondary reagents to detect mouse Frizzled-3. The detection limit for rmFrizzled-3 is ~0.5 ng/lane under non-reducing and reducing conditions. Flow Cytometry: Dilute this antibody to 0.2 mg/mL and add 10 μL of the diluted solution to 1-2.5x10^5 cells in a total reaction volume not exceeding 200 μL. The binding of the biotinylated monoclonal antibody may be visualized by adding streptavidin conjugated to a fluorochrome. Immunocytochemistry: Use with the appropriate secondary reagents at 8 μg/mL to detect Frizzled-3 in cells or frozen mouse embryos. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. -
说明
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Target Species of Antibody: Mouse
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 1 mL sterile PBS
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浓度
- Lot specific
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缓冲液
- Lyophilized from PBS, BSA
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
- 4°C or -20°C, Avoid freeze-thaw cycles.
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- FZD3 (Frizzled Family Receptor 3 (FZD3))
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别名
- FZD3 / Frizzled 3
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背景
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Name/Gene ID: FZD3
Subfamily: Frizzled
Family: GPCR
Synonyms: FZD3, Frizzled family receptor 3, Frizzled 3, HFz3, Frizzled homolog 3, Fz-3, Wnt receptor frizzled-3, Frizzled-3 -
基因ID
- 7976
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UniProt
- Q9NPG1
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途径
- WNT signaling, Tube Formation
抗原
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