FZD3 抗体 (Cysteine-Rich Domain, Extracellular)
Quick Overview for FZD3 抗体 (Cysteine-Rich Domain, Extracellular) (ABIN470847)
抗原
See all FZD3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Cysteine-Rich Domain, Extracellular
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特异性
- Recognizes mouse Frizzled-3. Shows no cross-reactivity with rmFrizzled-1, -4, -7 or -8. Species cross-reactivity: Human.
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纯化方法
- Protein G purified
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免疫原
- NS0-derived rmFrizzled-3 extracellular CRD domain.
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亚型
- IgG2a
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anti-Frizzled Family Receptor 3 (FZD3) (AA 121-220) antibody
FZD3 适用: 人, 小鼠, 大鼠, 非洲爪蟾 WB, ELISA, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Frizzled Family Receptor 3 (FZD3) (AA 23-205) antibodyFZD3 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Frizzled Family Receptor 3 (FZD3) (N-Term) antibodyFZD3 适用: 人, 小鼠, 大鼠, Bat, Cow, 犬, Hamster, 马, 猴, Pig, 兔, 小鸡 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Frizzled Family Receptor 3 (FZD3) (AA 55-157) antibodyFZD3 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2H5 unconjugated
anti-Frizzled Family Receptor 3 (FZD3) (AA 55-157) antibodyFZD3 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1B8 unconjugated
anti-Frizzled Family Receptor 3 (FZD3) (Internal Region) antibodyFZD3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Frizzled Family Receptor 3 (FZD3) (Internal Region) antibodyFZD3 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Frizzled Family Receptor 3 (FZD3) (AA 55-157) antibodyFZD3 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Frizzled Family Receptor 3 (FZD3) (AA 581-596), (Intracellular) antibodyFZD3 适用: 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Frizzled Family Receptor 3 (FZD3) (AA 481-530) antibodyFZD3 适用: 人, 小鼠 WB, ELISA, IHC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: ELISA, Flo, IHC, IHC-Fr, WB
Usage: Suitable for use in Direct ELISA, Western Blot, Flow Cytometry and Immunohistochemistry. By Flow Cytometry and Immunohistochemistry, this antibody detects mouse Frizzled-3 on differentiated embryoid bodies derived from the mouse embryonic cell line, D3. Flow Cytometry: Dilute this antibody to 5-10 μg/mL and add 10 mL of the diluted solution to 1-2.5x10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 μL of a 25 μg/mL stock solution of a secondary developing reagent such as goat anti-rat IgG conjugated to a fluorochrome. Immunohistochemistry: A biotin conjugate of this antibody at a concentration of 8 μg/mL was used to detect Frizzled-3 in frozen mouse embryo sections. Western Blot: Use at 1-2 μg/mL with the appropriate secondary reagents to detect mouse Frizzled-3. Using a colorimetric detection system, the detection limit for rmFrizzled-3 is ~1 ng/lane under non-reducing and reducing conditions. -
说明
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Target Species of Antibody: Mouse
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 200 μL sterile PBS
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浓度
- Lot specific
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缓冲液
- Lyophilized from PBS, 5 % trehalose.
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注意事项
- Avoid frequent freeze thaws. Store undiluted.
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储存条件
- -20 °C
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储存方法
- Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Reconstituted product is stable for 1 year at -20°C.
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- FZD3 (Frizzled Family Receptor 3 (FZD3))
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别名
- FZD3 / Frizzled 3
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背景
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Name/Gene ID: FZD3
Subfamily: Frizzled
Family: GPCR
Synonyms: FZD3, Frizzled family receptor 3, Frizzled 3, HFz3, Frizzled homolog 3, Fz-3, Wnt receptor frizzled-3, Frizzled-3 -
基因ID
- 7976
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UniProt
- Q9NPG1
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途径
- WNT signaling, Tube Formation
抗原
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