IL-4 抗体 (Biotin)
Quick Overview for IL-4 抗体 (Biotin) (ABIN465884)
抗原
See all IL-4 (IL4) 抗体适用
宿主
克隆类型
标记
应用范围
-
-
特异性
- Mouse IL-4
-
纯化方法
- Immunoaffinity purified
-
免疫原
-
E.coli derived recombinant Murine IL-4.
Type of Immunogen: Cell extract
-
-
-
-
应用备注
-
Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)
Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect Murine IL-4 by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Murine IL-4. To detect Murine IL-4 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Murine IL-4 (LS-C104801) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Murine IL-4. Western Blot: To detect Murine IL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for recombinant Murine IL-4 is 1.5-3 ng/lane, under either reducing or non-reducing conditions. -
说明
-
Target Species of Antibody: Mouse
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Sterile PBS containing 0.1 % BSA
-
浓度
- Lot specific
-
缓冲液
- Lyophilized from PBS, pH 7.2
-
注意事项
- Avoid freeze/thaw cycles.
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
-
-
- IL-4 (IL4) (Interleukin 4 (IL4))
-
别名
- IL4
-
背景
-
Name/Gene ID: IL4
Family: Hormone
Synonyms: IL4, B cell growth factor 1, B-cell stimulatory factor 1, B_cell stimulatory factor 1, BCGF1, BSF-1, Binetrakin, BCGF-1, Interleukin 4, Interleukin-4, Pitrakinra, BSF1, IL-4 -
基因ID
- 3565
-
UniProt
- P05112
-
途径
- JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation
抗原
-